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目的采用RP-HPLC测定清热二十五味丸中的栀子苷。方法色谱柱为Hypersil BDS C18柱,流动相为0.01%三乙胺(10%磷酸调pH7)-乙腈(87∶13),流速1.0 mL.min-1,检测波长240 nm。结果栀子苷10～100 mg.L-1与峰面积呈良好的线性,平均加样回收率为99.0%,RSD=0.93%。结论所用方法简便、快速、准确,适用于清热二十五味丸中栀子苷的质量控制。
Objective To determine geniposide in Twenty-five Flavors in Qingre Twenty-five Flavors by RP-HPLC. METHODS The column was Hypersil BDS C18 with the mobile phase consisted of 0.01% triethylamine (adjusted to pH7 with 10% phosphoric acid) -acetonitrile (87:13) at a flow rate of 1.0 mL · min-1 with a detection wavelength of 240 nm. Results Geniposide 10 ~ 100 mg.L-1 and peak area showed a good linearity, the average recovery was 99.0%, RSD = 0.93%. Conclusion The method is simple, rapid and accurate and is suitable for the quality control of Geniposide in Twenty-five Taste of Qingre.