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以尼龙网作载体，用戊二醛交联将葡萄糖氧化酶（ＧＯＤ）固定装成酶柱并联结于流动体系中，采用流动注射分光光度法研究磁场对固定化ＧＯＤ催化活性的影响。结果表明，在０℃、５℃、４５℃下以１．０×１０－２ｍｏｌ·Ｌ－１的葡萄糖为底物及５５℃下以０．１ｍｏｌ·Ｌ－１的葡萄糖为底物时，０．２２Ｔ静磁场能显著提高固定化ＧＯＤ的反应速度，且磁场效应与底物浓度及磁场作用时间有关。用动力学方法考察了不同强度的磁场对溶解ＧＯＤ反应进程的影响，未发现磁场效应。初步提出了酶反应系统处于热力学非平衡态是产生磁场效应的前提
Nylon mesh was used as the carrier. Glucose oxidase (GOD) was immobilized on the mobile phase by glutaraldehyde cross-linking. The effect of magnetic field on the catalytic activity of immobilized GOD was investigated by flow injection spectrophotometry. The results showed that when glucose was 1.0 × 10-2 mol·L-1 at 0 ℃, 5 ℃ and 45 ℃ and substrate was glucose at 0.1 mol·L-1 at 55 ℃, 0 .22T static magnetic field can significantly improve the reaction rate of immobilized GOD, and the magnetic field effect and substrate concentration and magnetic field time. The effect of magnetic field with different intensities on the reaction of dissolved GOD was investigated by kinetic method, and no magnetic field effect was found. It is preliminarily proposed that the enzyme reaction system is in thermodynamic non-equilibrium state and is a magnetic field effect