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目的观察金丝桃素对急性弓形虫感染小鼠脾脏CD4+、CD8+T淋巴细胞亚群的影响。方法将40只昆明种雌性小鼠随机分为4组:空白对照组(A组)、弓形虫感染组(B组)、金丝桃素对照组(C组)和金丝桃素治疗组(D组),每组各10只小鼠。除A组和C组外,B、D两组每组小鼠腹腔接种RH株弓形虫速殖子1×103个,建立急性弓形虫感染动物模型。C、D组于感染12h后腹腔注射给药,A、B组同时进行生理盐水腹腔注射,连续7d后,用流式细胞仪检测小鼠脾脏中CD4+、CD8+T淋巴细胞水平。结果C组和D组CD4+T细胞百分率分别为20.57±1.76和23.33±1.82,CD4+与CD8+T细胞比值分别为2.04±0.40和2.29±0.65,A、B组CD4+T百分率及CD4+/CD8+值分别为12.13±2.35、15.96±2.71和1.35±0.30、0.77±0.92,差异有统计学意义(P<0.01);A、B、C、D组CD8+T细胞百分率分别为8.95±0.40、20.74±1.50、10.42±2.43和10.86±3.18,差异有统计学意义(P<0.01)。结论金丝桃素可显著改善急性弓形虫感染小鼠的细胞免疫功能,增强小鼠抗弓形虫感染的能力。
Objective To investigate the effect of hypericin on the subsets of CD4 + and CD8 + T lymphocytes in mice infected with Toxoplasma gondii. Methods Forty Kunming female mice were randomly divided into 4 groups: blank control group (A group), Toxoplasma gondii infection group (B group), hypericin control group (C group) and hypericin treatment group Group D), 10 mice in each group. Except group A and group C, 1 × 103 Tachyzoites tachyzoites were inoculated intraperitoneally into groups B and D, respectively. Animal models of acute Toxoplasma gondii infection were established. Groups C and D were intraperitoneally injected 12h after infection. Groups A and B received intraperitoneal injections of physiological saline at the same time. After 7 days, the levels of CD4 + and CD8 + T lymphocytes in spleens were detected by flow cytometry. Results The percentage of CD4 + T cells in group C and group D were 20.57 ± 1.76 and 23.33 ± 1.82 respectively, the ratios of CD4 + to CD8 + T cells were 2.04 ± 0.40 and 2.29 ± 0.65, respectively. The percentage of CD4 + T and CD4 + / CD8 + The values were 12.13 ± 2.35,15.96 ± 2.71 and 1.35 ± 0.30,0.77 ± 0.92, the difference was statistically significant (P <0.01); the percentage of CD8 + T cells in groups A, B, C and D were 8.95 ± 0.40 and 20.74 ± 1.50, 10.42 ± 2.43 and 10.86 ± 3.18 respectively, the difference was statistically significant (P <0.01). Conclusion Hypericin can significantly improve the cellular immune function in mice infected with Toxoplasma gondii and enhance the ability of anti-Toxoplasma infection in mice.