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目的 探讨 CD4 4v6 、ki- 6 7在宫颈癌中的表达情况及其临床意义。 方法 应用免疫组织化学 SP法检测 6 3例宫颈癌和正常宫颈组织中 CD4 4v6 、ki- 6 7的表达情况并分析其与有关的临床病理因素间的关系。 结果 CD4 4v6 的着色部位主要在细胞膜和细胞质 ;而 ki- 6 7主要在胞核。 CD4 4v6 在正常宫颈上皮、宫颈原位癌和宫颈浸润癌中的阳性表达情况分别为 0 / 10 ,2 / 6和 37/ 5 7;ki- 6 7分别为 0 / 10 ,4 / 6和 5 6 / 5 7。随着病程进展 ,CD4 4v6 和 ki- 6 7的阳性表达率显著升高 (P<0 .0 1)。 CD4 4v6 阳性表达与宫颈癌的盆腔淋巴结转移、脉管浸润和 ki- 6 7的表达情况有关 (P<0 .0 5 ) ;而与临床分期、分化程度、组织学类型、间质浸润和癌灶大小无关 (P>0 .0 5 )。有淋巴结转移、脉管浸润和ki- 6 7过表达者 CD4 4v6 的阳性表达率显著高于无淋巴结转移、脉管浸润和 ki- 6 7低表达者。 结论 CD4 4v6 的阳性表达可能在宫颈癌的发生发展、淋巴结转移、脉管浸润和癌细胞增殖过程中起着重要的作用 ,但非唯一决定因素。CD4 4v6 的检测对于了解宫颈癌的生物学行为和判断宫颈癌患者的预后有一定的实用价值
Objective To investigate the expression of CD4 4v6 and Ki-67 in cervical cancer and its clinical significance. Methods Immunohistochemical SP method was used to detect the expression of CD4 4v6 and Ki-67 in 63 cases of cervical cancer and normal cervical tissue and to analyze its relationship with clinicopathological factors. Results The staining site of CD4 4v6 was mainly in the plasma membrane and cytoplasm, while ki- 6 7 was mainly in the nucleus. The positive expression rates of CD4 4v6 in normal cervical epithelium, cervical carcinoma in situ and invasive cervical carcinoma were 0/10, 2/6 and 37/570, respectively. The positive rates of Ki-67 were 0/10, 4/6 and 5, respectively 6/5 7. With the progression of the disease, the positive rates of CD4 4v6 and Ki-67 were significantly increased (P <0.01). The positive expression of CD4 (4v6) was correlated with pelvic lymph node metastasis, vascular invasion and expression of Ki-67 in cervical cancer (P <0.05), but not correlated with clinical stage, degree of differentiation, histological type, interstitial infiltration and cancer The size of the stove had nothing to do (P> 0.05). The positive rate of CD4 4v6 in patients with lymph node metastasis, vascular invasion and overexpression of Ki-67 was significantly higher than those without lymph node metastasis, vascular invasion and low Ki-67 expression. Conclusion The positive expression of CD4 4v6 may play an important role in the development of cervical cancer, lymph node metastasis, vascular invasion and cancer cell proliferation, but not the only determinant. CD4 4v6 detection for understanding the biological behavior of cervical cancer and to determine the prognosis of cervical cancer patients have some practical value