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目的探查三黄抗氧化方抑制乳腺癌MCF-7细胞氧化应激与增殖的疗效。方法将实验分为空白组:MCF-7细胞培养;实验组按黄芪、制大黄、片姜黄比例分:实验组1∶1∶1∶1,实验组2∶3∶1∶1,实验组3∶6∶1∶1;氧化应激组:以过氧化氢模拟细胞氧化应激状态;乙酰谷氨酸液(NAC)还原组:氧化应激组中加入NAC还原应激反应;免疫荧光法评价其抗MCF-7细胞产生反应性氧化物疗效及ELISA法评价氧化应激后细胞培养液中VEGF含量;同时MTT法评价三黄抗氧化方抑制MCF-7细胞的增殖率。结果实验组2抗氧化氧化应激结果优于其他两组,并显著降低细胞培养液中VEGF水平;同时各实验组MCF-7细胞增殖率得到显著抑制并呈现明显的浓度依耐性,相对空白组,实验组1~3在8mg/ml浓度下分别抑制MCF-7细胞增殖达57%、67%及56%,实验组2的MCF-7细胞增殖抑制率显著高于实验组1与3,有统计学差异(P<0.01)。结论三黄抗氧化方抑制MCF-7细胞氧化应激同时抑制其增殖率,并降低氧化应激引起的VEGF表达。
Objective To investigate the effect of Sanhuang Antioxidant on inhibiting oxidative stress and proliferation of breast cancer MCF-7 cells. Methods The experiment was divided into blank group: MCF-7 cell culture; experimental group according to astragalus, rhubarb, turmeric ratio: experimental group 1: 1: 1: 1, experimental group 2: 3: 1: 1, experimental group 3 : 6: 1: 1; oxidative stress group: hydrogen peroxide simulated cell oxidative stress; acetyl glutamate solution (NAC) reduction group: oxidative stress group added NAC reduction stress response; The anti-MCF-7 cells produce reactive oxygen species curative effect and the ELISA method to evaluate the VEGF content in the cell culture medium after oxidative stress; At the same time, MTT method evaluated that the Sanhuang Antioxidant Prescription inhibited the proliferation rate of the MCF-7 cells. Results The antioxidant oxidative stress in experimental group 2 was better than the other two groups, and significantly decreased the level of VEGF in cell culture medium. At the same time, the proliferation rate of MCF-7 cells in each experimental group was significantly inhibited and showed obvious concentration-dependent tolerance. The relative blank group , The experimental groups 1 to 3 inhibited the proliferation of MCF-7 cells by 57%, 67% and 56% respectively at the concentration of 8mg / ml, and the inhibition rate of MCF-7 cells in experimental group 2 was significantly higher than that of experimental groups 1 and 3 Statistical difference (P <0.01). Conclusion Sanhuang Antioxidant can inhibit the oxidative stress of MCF-7 cells and inhibit its proliferation rate and reduce the expression of VEGF induced by oxidative stress.