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An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the ef fects of protein to energy ratio on growth, for the rare minnow( Gobiocypris rarus), which are critical to nutrition standardization for model fi sh. Twenty-four diets were formulated to contain three gross energy(10, 12.5, 15 kJ/g), four protein(20%, 25%, 30%, 35%), and two lipid levels(3%, 6%). The results showed that optimal dietary E/P was 41.7–50 kJ/g for maximum growth in juvenile rare minnows at 6% dietary crude lipid. At 3% dietary lipid, specifi c growth rate(SGR) increased markedly when E/P decreased from 62.5 kJ/g to 35.7 kJ/g and gross energy was 12.5 kJ/g, and from 75 kJ/g to 42.9 kJ/g when gross energy was 15.0 kJ/g. The optimal gross energy was estimated at 12.5 kJ /g and excess energy decreased food intake and growth. Dietary lipid exhibited an apparent protein-sparing eff ect. Optimal protein decreased from 35% to 25%–30% with an increase in dietary lipid from 3% to 6% without adversely ef fecting growth. Dietary lipid level af fects the optimal dietary E/P ratio. In conclusion, recommended dietary protein and energy for rare minnow are 20%–35% and 10–12.5 k J/g, respectively.
An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the ef fects of protein to energy ratio on growth, for the rare minnow (Gobiocypris rarus), which are critical to nutrition standardization for model fi sh Twenty-four diets were formulated to contain three gross energy (10, 12.5, 15 kJ / g), four protein (20%, 25%, 30%, 35% . The results showed that optimal dietary E / P was 41.7-50 kJ / g for maximum growth in juvenile rare minnows at 6% dietary crude lipid. At 3% dietary lipid, specifi c growth rate (SGR) increased markedly when E / P was 41.7-50 kJ / decreased from 62.5 kJ / g to 35.7 kJ / g and gross energy was 12.5 kJ / g, and from 75 kJ / g to 42.9 kJ / g when gross energy was 15.0 kJ / g. The optimal gross energy was estimated at 12.5 kJ / g and excess energy decreased food intake and growth. Dietary lipid tested an apparent protein-sparing effct. Optimal protein decreased from 35% to 25% -30% with an increase in dietary lipid from 3% to 6% without adversely ef fecting growth. Dietary lipid level af fects the optimal dietary E / P ratio. In conclusion, recommended dietary protein and energy for rare minnow are 20% -35% and 10-12.5 kJ / g, respectively.