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目的:探讨茶多酚对大鼠睾丸扭转/复位模型的保护作用。方法:将24只健康雄性Wistar大鼠随机分为3组,每组8只。第Ⅰ组为假手术组(切开左侧阴囊游离睾丸,但不予扭转),第Ⅱ、Ⅲ组扭转左侧睾丸720°6h,分别于扭转复位前30min腹腔注射生理盐水和茶多酚,术后连续3d分别以低剂量维持。3组大鼠喂养至术后第5天处死,切取左侧扭转睾丸检测睾丸组织中超氧化物歧化酶(SOD)和丙二醛(MDA)含量;以原位缺口末端标记法(TUNEL)检测生精细胞凋亡指数(AI)。结果:Ⅰ、Ⅱ、Ⅲ3组左侧扭转睾丸组织SOD活力分别为(285.00±22.51)、(242.00±17.62)、(261.00±10.01)nU/mg;MDA含量分别为(1.81±0.20)、(4.34±0.34)、(2.94±0.38)nmol/mg;3组之间比较均有显著性差异。Ⅰ、Ⅱ、Ⅲ3组左侧扭转睾丸生精细胞凋亡指数(AI)分别为6.64±1.82、55.23±6.46、31.84±5.56,第Ⅲ组与第Ⅱ组相比,其生殖细胞凋亡明显减少(P<0.05)。结论:茶多酚对因睾丸扭转导致的缺血再灌注损伤具有保护作用。
Objective: To investigate the protective effect of tea polyphenols on testicular torsion / detorsion in rats. Methods: Twenty-four healthy male Wistar rats were randomly divided into 3 groups with 8 rats in each group. Group I was sham operation group (incision of the left scrotum free testis, but not reverse), the group Ⅱ, Ⅲ reverse left testicular 720 ° 6h, respectively, 30 minutes before the reverse rotation of saline and polyphenols injected intraperitoneally, After 3 consecutive days were maintained at low doses. The rats in the three groups were sacrificed on the 5th day after operation and the contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in the testis tissue were detected by reverse torsion testis. TUNEL Apoptosis index (AI). RESULTS: The SOD activities of the testis in the left torsion group were (285.00 ± 22.51), (242.00 ± 17.62) and (261.00 ± 10.01) nU / mg respectively in the groups Ⅰ, Ⅱ and Ⅲ3, and were 1.81 ± 0.20 and 4.34 ± 0.34) and (2.94 ± 0.38) nmol / mg, respectively. There were significant differences among the three groups. The apoptotic index (AI) of spermatogenic cells on the left side in Ⅰ, Ⅱ and Ⅲ groups were 6.64 ± 1.82, 55.23 ± 6.46 and 31.84 ± 5.56, respectively. The apoptosis rate of germ cells in group Ⅲ was significantly lower than that in group Ⅱ (P <0.05). Conclusion: Tea polyphenols have a protective effect on ischemia-reperfusion injury caused by testicular torsion.