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本文对镍处理下高冰草幼苗生长,茎部和根部丙二醛(MDA)含量,可溶性蛋白含量,超氧化物岐化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性及叶片光合色素含量的变化进行了研究。结果表明,低浓度镍(10μmol.L-1)对高冰草幼苗生长无显著抑制效应,但随镍浓度增加各项生长指标显著减少,且镍对高冰草幼苗根部损伤更为显著。光合色素含量随镍浓度升高呈减少趋势。高冰草茎部和根部MDA含量与镍浓度呈显著正相关关系。根部可溶性蛋白含量随镍浓度升高呈增加趋势。低浓度镍处理(10μmol.L-1)下,SOD、POD和CAT活性均有所提高,保护酶系统处于正常状态,但随镍浓度升高,3种保护酶活性受到不同程度抑制,保护酶系统平衡被破坏。
In this paper, the growth, the contents of malondialdehyde (MDA), soluble protein, superoxide dismutase (SOD), peroxidase (POD) and catalase CAT activity and leaf photosynthetic pigment content were studied. The results showed that the low concentration of nickel (10μmol·L-1) had no significant inhibitory effect on the growth of P. soongorica seedlings. However, all the growth indexes were significantly decreased with the increase of nickel concentration. Photosynthetic pigment content decreased with increasing nickel concentration. There was a significant positive correlation between the content of MDA and the concentration of nickel in the stem and root of Populus euphratica. The root soluble protein content increased with increasing nickel concentration. The activity of SOD, POD and CAT increased at low concentration of nickel (10μmol.L-1), and the protective enzyme system was in normal condition. However, with the increase of nickel concentration, the activities of three protective enzymes were inhibited to some extent, System balance is destroyed.