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目的:观察非诺贝特对大鼠勃起功能和睾酮水平的影响,探讨非诺贝特致勃起功能障碍(ED)的原因。方法:30只雌性SD大鼠去势2周后分别在交配实验前48h和4h皮下注射苯甲酸雌二醇20μg和黄体酮500μg。将30只雄性大鼠随机分为2组:非诺贝特组和对照组,每组15只。非诺贝特组大鼠用非诺贝特31.5mg/kg溶于蒸馏水中(总体积4mL)灌胃,1次/d,连续14d;对照组大鼠用同等容积蒸馏水灌胃,1次/d,连续14d。在停药后第1天行交配实验,观察记录30min内2组大鼠的骑跨次数(MF)、插入次数(IF)、骑跨潜伏期(ML)和射精潜伏期(EL),并用放射免疫法检测血清和阴茎组织中睾酮水平。结果:非诺贝特组大鼠的MF和IF分别为(9.45±1.65)和(4.15±1.38)次/min、ML和EL分别为(14.1±7.0)和(5.3±5.9)min,对照组大鼠上述指标分别为(31.23±3.85)和(12.80±3.02)次/min、(6.2±4.3)和(16.2±6.0)min,差异有统计学意义(均P<0.05);大鼠血清和阴茎组织的睾酮水平非诺贝特组分别为(51.26±34.15)和(62.57±46.37)nmol/L,对照组分别为(153.72±83.93)和(164.89±94.02)nmol/L,差异有统计学意义(均P<0.05)。结论:非诺贝特能降低大鼠的性功能和睾酮水平。非诺贝特引致睾酮水平降低可能是勃起功能障碍的主要原因之一。
OBJECTIVE: To observe the effect of fenofibrate on erectile function and testosterone in rats and explore the cause of fenofibrate-induced erectile dysfunction (ED). Methods: Thirty female SD rats were injected subcutaneously with 20 μg of estradiol benzoate and 500 μg of progesterone subcutaneously 48 h and 4 h before ovulation respectively after 2 weeks of ovariectomy. Thirty male rats were randomly divided into two groups: fenofibrate group and control group, 15 rats in each group. Rats in fenofibrate group were dosed with fenofibrate 31.5mg / kg in distilled water (total volume 4mL) once a day for 14 days. Rats in control group were intragastrically administrated with the same volume of distilled water once a day, d, continuous 14d. Mild mating test was performed on the first day after drug withdrawal. The number of rides (MF), insertion frequency (IF), riding latency (ML) and ejaculation latency (EL) Test serum and penile tissue testosterone levels. Results: The MF and IF of fenofibrate group were (9.45 ± 1.65) and (4.15 ± 1.38) / min respectively, the ML and EL were (14.1 ± 7.0) and (5.3 ± 5.9) min, respectively. The control group The above indexes of rats were (31.23 ± 3.85) and (12.80 ± 3.02) times / min, (6.2 ± 4.3) and (16.2 ± 6.0) min, respectively, with statistical significance (all P <0.05) The levels of testosterone in penile tissue were (51.26 ± 34.15) and (62.57 ± 46.37) nmol / L, respectively, and those in control group were (153.72 ± 83.93) and (164.89 ± 94.02) nmol / L, Significance (all P <0.05). Conclusion: Fenofibrate can reduce sexual function and testosterone levels in rats. Decreased levels of testosterone by fenofibrate may be one of the major causes of erectile dysfunction.