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近十多年来,发现麻疹病毒与中枢神经系统某些慢病毒感染(如亚急性硬化性全脑炎与脑脊髓多发性硬化症)有关,因而重新激起了对该病毒的兴趣。作者以麻疹病毒Hallé株免疫BALB/c小鼠,然后取脾细胞与SP2/OAg14骨髓瘤细胞融合,产生抗-P(多聚酶)、抗-NP(核蛋白)、抗-HA(血凝素)、抗-L(彩聚酶)和抗-M(基质)单克隆抗体。急性(溶细胞性)或持续性感染麻疹病毒的BGM(非洲绿猴肾)细胞,用丙酮室温固定10分钟或不固定悬于磷
For nearly a decade, the discovery that measles virus is associated with certain lentiviral infections in the central nervous system, such as sub-acute sclerosing total encephalitis and multiple sclerosis of the brain and spinal cord, has revived interest in the virus. The BALB / c mice were immunized with Hallé strain of measles virus and then spleen cells were fused with SP2 / OAgl4 myeloma cells to produce anti-P (polymerase), anti-NP (nucleoprotein), anti-HA , Anti-L (chromaffin) and anti-M (matrix) monoclonal antibodies. Acute (cytolytic) or persistent measles virus BGM (African green monkey kidney) cells were fixed with acetone for 10 minutes at room temperature or left undistracted