玉米大斑病菌黑色素合成酶基因4HNR的克隆及其启动子预测

• 作 者: 温雷蕾 曹志艳 董金皋
• 机 构: 河北农业大学植物保护学院植物分子病理学实验室,
• 出 处: 植物病理学报
• 发表日期: 2008年06期
• 关键词: 玉米大斑病菌 黑色素 合成酶基因 克隆 启动子预测 附着胞产生 机械压力 寄主 病原真菌 致病性 作用 组织 影响 丝状 利用 颗粒 甘油 发育

4HNR(1,3,6,8-tetra-HN reductase)gene of melanin biosynthesis in Setosphaeria turcica had been cloned successfully by RT-PCR in this study.Both sequences of DNA and cDNA of 4HNR were 807 bp and there was no intron in the sequences.This gene only had single copy in genome through Southern blotting analysis.A 2 285 bp for the flanking sequence of 5’ had been obtained and it had promoter structure through the software analysis.

4HNR (1,3,6,8-tetra-HN reductase) gene of melanin biosynthesis in Setosphaeria turcica had been cloned successfully by RT-PCR in this study. Bth sequences of DNA and cDNA of 4HNR were 807 bp and there was no intron in the sequences. This gene only had single copy in genome through Southern blotting analysis. A 2 285 bp for the flanking sequence of 5 ’had been obtained and it had promoter structure through the software analysis.