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本试验用大麦(品系78132)为材料。用 EMS 和γ射线进行诱变处理,并用咖啡因作后处理。观察了 M_1代生理损伤和叶色变异嵌合现象及 M_2代叶绿素突变。实验结果表明,0.1M 和0.3M EMS 大大降低 M_1代植株结实率和分蘖率;EMS 处理 M_2代叶色变异嵌合现象可高达30%,M_2代叶绿素突变率可高达20%(M_1穗计)或7%(M_2苗计)。24kRγ射线诱发 M_1代叶色变异现象出现率约2.4%,M_2代叶绿素突变约7.6%(M_1穗计)或1.8%(M_2苗计)。咖啡因后处理不增加 EMS 处理的突变率,但增加γ射线处理的突变率。咖啡因对γ射线诱发叶绿素突变的增效作用与浓度有关,4mM 咖啡因后处理效果较佳。
In this experiment, barley (strain 78132) was used as material. Mutagenic treatment with EMS and gamma rays and post-treatment with caffeine. The physiological and leaf chromatic aberration chimerism in M_1 generation and the chlorophyll mutation in M_2 generation were observed. The results showed that 0.1M and 0.3M EMS significantly reduced the seed setting rate and tillering rate of M_1 generation. The leaf color variation chimerism in M_2 generation was up to 30%, and the M_2 chlorophyll mutation rate was as high as 20% Or 7% (M_2 seed count). The occurrence rate of leaf color variation of M_1 generation induced by 24kRγ ray was about 2.4%, the chlorophyll mutation of M_2 generation was about 7.6% (M_1 panicle) or 1.8% (M_2). Caffeine post-treatment does not increase the mutation rate of EMS treatment, but increases the mutation rate of gamma-ray treatment. Caffeine on γ-ray-induced chlorophyll mutation synergistic effect and concentration, 4mM caffeine post-treatment better.