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在碱性溶液中 ,盐酸麻黄素与硫酸铜生成蓝紫色络合物 ,其最大吸收波长为 60 1 .2 nm。且 D-型盐酸伪麻黄素显色灵敏度大于 L-型。标准曲线绘制表明 ,显色线性范围为 0 .5～ 3 mg/ ml。三批 D-盐酸伪麻黄素回收率分别为 98.54%± 3 .3 2 %、99.54%± 2 .3 6%及 1 0 1 .4 9%± 1 .2 1 % ;三批 L-盐酸麻黄素回收率分别为 98.55%± 1 .3 2 %、1 0 1 .53 %± 1 .3 9%及 1 0 0 .2 7%± 1 .69% ,表明本测定法准确性与稳定性良好。酶工程技术制备的三批 L-盐酸麻黄素测定结果 ,其含量分别为 1 0 1 .4 0 %、98.3 0 %及 1 0 3 .1 8%。该法除用于测定药用麻黄素外 ,尤其适用于化学合成及麻黄草抽提液中麻黄素及其盐类的定性定量分析。
In alkaline solution, ephedrine hydrochloride and copper sulfate generate blue-purple complexes, the maximum absorption wavelength of 60 1 .2 nm. And D-type pseudoephedrine hydrochloride color development sensitivity than L-type. The standard curve shows that the linear range of color development is 0.5 ~ 3 mg / ml. The recoveries of three batches of pseudoephedrine hydrochloride were 98.54% ± 3 .3 2%, 99.54% ± 2.36% and 1 0 1 .4 9% ± 1. 21%, respectively. The three batches of L- The recoveries were 98.55% ± 1.3.2%, 100.153% ± 1.39% and 100.0.27% ± 1.69%, respectively, indicating the accuracy and stability of this assay . Enzyme engineering prepared three batches of L-hydrochloric acid ephedrine determination, the content was 1 0 1 .4 0%, 98.3 0% and 1 0 3. In addition to the method for the determination of medicinal ephedrine, especially for chemical synthesis and ephedrine extract of ephedrine and its qualitative and quantitative analysis of salts.