慢性乙肝患者外周血Th1/Th2类细胞因子变化的动态观察

• 作 者: 陈凯红 蒋祥虎 蒲云川 杨美蓉 蒋跃明
• 机 构: 无锡市传染病医院传染病科,无锡市传染病医院传染病科,无锡市传染病医院传染病科,无锡市传染病医院传染病科,无锡市传染病医院传染病科 无锡214005,无锡214005,无锡214005,无锡214005
• 出 处: 临床消化病杂志
• 发表日期: 2006年06期
• 关键词: 慢性乙肝 IL-2 IFN-γ IL-4 IL-10

目的探讨慢性乙肝患者Th1/Th2类细胞因子变化的意义。方法采用双抗夹心ELISA法对34例慢性乙肝患者发病时和治疗后肝功能恢复正常时外周血Th1类细胞因子(IL-2、IFN-γ)及Th2类细胞因子(IL-4、IL-10)进行检测。与此同时检测血清HBV-DNA。结果与正常对照组相比,IL-2显著下降,下降程度在慢肝轻度<慢肝中度<慢肝重度(P<0.05和0.001);IL-10在慢肝中、重度显著升高(P<0.01),升高程度在慢肝重度>慢肝中度>慢肝轻度,治疗后慢肝中度和慢肝重度较治疗前显著下降(P<0.05)。在慢肝中、重度HBVDNA量治疗后较治疗前显著减少。IL-2、IFN-γ及IL-4、IL-10的水平变化与HBVDNA量无显著相关性。结论慢性乙型肝炎发病时Th2细胞功能增强,且病情越重,Th2细胞功能越强;肝功恢复正常后,Th2细胞功能下降,HBVDNA量减少。 Objective To investigate the significance of Th1 / Th2 cytokines in chronic hepatitis B patients. Methods Thirty-two patients with chronic hepatitis B were enrolled in this study. The levels of Th1 cytokines (IL-2, IFN-γ) and Th2 cytokines (IL-4 and IL- 10) for testing. At the same time, serum HBV-DNA was detected. Results Compared with the normal control group, the level of IL-2 was significantly decreased, with the degree of decrease being mild in chronic liver moderate to moderate> moderate to moderate, and the levels of moderate and slow liver were decreased significantly after treatment (P <0.05). In the slow liver, the amount of severe HBVDNA was significantly reduced after treatment than before treatment. The levels of IL-2, IFN-γ, IL-4 and IL-10 had no significant correlation with the level of HBVDNA. Conclusion The function of Th2 cells is enhanced in patients with chronic hepatitis B, and the more severe the disease, the stronger the function of Th2 cells. After the liver function returns to normal, the function of Th2 cells decreases and the amount of HBVDNA decreases.