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目的:探讨复方中药脑尔康对小鼠学习记忆障碍的保护作用及其对胆碱酯酶活性的影响及保护神经元的抗痴呆作用机制。方法:健康昆明种雄性小鼠60只,按随机数字表法分为6组,除空白组外,连续造模50d后,每组随机取3只,用苏木精-伊红(HE)染色法,观察小鼠海马CA1区细胞数量及形态,其余动物迅速断头取脑,冰台分离海马及皮质,检测各自的胆碱酯酶活性。结果:模型组胆碱酯酶活性明显增高犤脑皮质、海马:模型组(3.0±0.1),(2.7±0.1)mol/s;空白组(2.2±0.1),(2.2±0.1)mol/s犦(q=16.7,48.2,P<0.01),用药各组与模型组比较,除小剂量组外胆碱酯酶活性均明显低于模型组(q=7.9～37.9,P<0.01);模型组犤(47±4)个/200μm犦海马CA1区神经元数量明显少于空白组犤(101±11)个/200μm犦(q=5.39,P<0.01),可见神经元脱失,并有胶质细胞增生。用药各组与模型组比较,仅有中药大、中剂量组神经元数量显著多于模型组(q=3.4,P<0.05或q=4.3,P<0.01)。结论:脑尔康能明显降低老年痴呆模型小鼠皮层及海马胆碱酯酶活性,并有保护海马CA1区神经元的作用。
OBJECTIVE: To investigate the protective effect of Naoer Kang, a compound Chinese herbal medicine, on learning and memory impairment in mice and its effect on cholinesterase activity and the protective mechanism of neurons against dementia. METHODS: Sixty Kunming male mice were randomly divided into 6 groups according to the random number table method. Except for the blank group, after continuous modeling for 50 days, 3 mice were randomly selected from each group and stained with hematoxylin-eosin (HE). Methods: The number and morphology of cells in the hippocampal CA1 region of the mouse were observed. The rest of the animals were quickly decapitated and the brains were removed. The hippocampus and cortex were separated on an ice table and their cholinesterase activity was measured. Results: The cholinesterase activity in the model group increased significantly in the camphor cortex and hippocampus: the model group (3.0±0.1), (2.7±0.1) mol/s; the blank group (2.2±0.1), (2.2±0.1) mol/s犦(q=16.7,48.2,P<0.01), compared with the model group, the activity of cholinesterase was lower than that of the model group except for the low-dose group (q=7.9～37.9, P<0.01); The number of neurons in the hippocampal CA1 region of the group (47±4)/200 μm was significantly lower than that in the blank group (101±11)/200 μm (q=5.39, P<0.01). Gliosis. Compared with the model group, only the number of neurons in the large and middle dose groups of Chinese medicine was significantly more than that in the model group (q=3.4, P<0.05 or q=4.3, P<0.01). Conclusion: Naoerkang can significantly reduce the activity of cholinesterase in cortex and hippocampus of Alzheimer’s disease model mice, and protect the neurons in hippocampal CA1 area.