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实验取经ＰＭＳＧ－ｈＣＧ处理的未成年雌性大鼠卵巢，用胶原酶－ＤＮＡ酶消化，制得黄体细胞悬浮液，预孵育１ｈ后加入各种处理因素，继续孵育２ｈ，用放射免疫方法测孵育液中孕酮的量。结果：孵育液中含有高钙或高钾或加入Ａ２３１８７时均可增加黄体细胞基础及ｈＣＧ诱导的孕酮生成量。相反，减少钙的浓度或加入ＥＧＡＴ或戊脉胺，孕酮生成量则明显减少。酪氨酸抑制ｈＣＧ刺激的孕酮生成，但对高钙、高钾和Ａ２３１８７增加孕酮的作用没有影响，并对上述三者分别与ｈＣＧ同时作用所致孕酮生成增加也没有影响。提示：大鼠黄体细胞孕酮生成依赖于细胞内外的钙；细胞内外钙浓度的变化不影响酪氨酸抗ｈＣＧ致孕酮生成作用；钙与ｈＣＧ使孕酮增加的作用可能是通过不同机制。
In the experiment, the ovaries of PMSG-hCG-treated female rats were digested with collagenase-DNAase to prepare luteal cell suspension. After pre-incubation for 1h, various treatment factors were added and the incubation continued for 2h. The incubation solution was measured by radioimmunoassay The amount of progesterone. Results: Incubation fluid containing high calcium or potassium or adding A23187 can increase the basis of corpus luteum and hCG-induced progesterone production. Conversely, decreasing calcium levels or adding EGAT or pentamine led to a significant reduction in progesterone production. Tyrosine inhibits hCG-stimulated progesterone production, but has no effect on the effects of high calcium, high potassium and A23187 on the progesterone uptake, nor does it have an effect on the progesterone production by the above three simultaneous actions with hCG, respectively. It is suggested that the progesterone production of rat luteal cells depends on the intracellular and extracellular calcium. The changes of intracellular and extracellular calcium concentrations do not affect tyrosine production of progesterone induced by hCG. The effect of calcium and hCG on progesterone increase may be through different mechanisms.