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设计并合成了两个新的氟代香豆素化合物8-(3-氟苯甲酰基)-7-羟基-4-甲基香豆素(3a)和8-(4-氟苯甲酰基)-7-羟基-4-甲基香豆素(3b),其结构通过元素分析、IR和1HNMR进行了表征,通过X-射线单晶衍射测定了化合物3b的晶体结构,单晶结构分析表明,化合物3b的晶体属于单斜晶系.体外抑菌试验结果显示,目标化合物对大肠杆菌(E.coli)、枯草杆菌(B.subtilis)和金色葡萄球菌(S.aureus)有不同程度的抑制作用;体外抗氧化实验显示,目标化合物对超氧阴离子自由基(O2-·)和二苯代苦味肼基自由基(DPPH·)均有良好的清除能力;运用荧光光谱法研究了不同温度下目标化合物与牛血清白蛋白(BSA)的相互作用,结果表明,目标化合物对BSA的荧光猝灭均属于静态猝灭,相关热力学参数表明,化合物3a(H<0,S<0,G<0)与BSA二者之间主要以氢键或范德华力相结合,化合物3b(H>0,S>0,G<0)与BSA二者之间主要靠疏水作用力相结合;依据Fster’s非辐射能量转移理论,求得BSA与化合物3a和化合物3b间的距离(r)分别为2.53和2.65nm,说明两个化合物与BSA之间可能发生了非辐射能量转移.
Two new fluoroumarin compounds, 8- (3-fluorobenzoyl) -7-hydroxy-4-methylcoumarin (3a) and 8- (4-fluorobenzoyl) The structure of compound 3b was characterized by elemental analysis, IR and 1HNMR. The crystal structure of compound 3b was determined by X-ray single crystal diffraction. The single crystal structure analysis showed that, The crystal of compound 3b belongs to the monoclinic system.In vitro antibacterial test results show that the target compounds have different inhibitory effects on E.coli, B. subtilis and S.aureus ; In vitro antioxidant experiments showed that the target compounds have good scavenging ability on superoxide anion radical (O2- ·) and dibenzylidenebromohydrazyl radical (DPPH ·); using fluorescence spectroscopy to study the effect of target The results showed that the fluorescence quenching of the target compound to BSA all belong to the static quenching. The related thermodynamic parameters indicated that the compound 3a (H <0, S <0, G <0) And BSA mainly by hydrogen bonds or van der Waals forces, compound 3b (H> 0, S> 0, G <0) and BSA mainly rely on the hydrophobic interaction between According to Fster’s theory of non-radiative energy transfer, the distances (r) between BSA and compounds 3a and 3b were found to be 2.53 and 2.65 nm, respectively, indicating that non-radiative energy transfer between the two compounds and BSA may occur.