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目的探讨过氧化物酶体增殖物激活型受体γ(PPARγ)及配体曲格列酮(Troglitazone,TGZ)对绒癌JEG-3细胞浸润能力的影响。方法通过免疫荧光细胞化学方法检测JEG-3细胞系中PPARγ的表达,采用Transwell侵入系统检测PPARγ的激动配体TGZ对绒癌细胞浸润能力的影响。结果在绒癌JEG-3细胞系中有PPARγ蛋白表达,PPARγ激动配体TGZ处理后浸润人工基底膜细胞数明显减少,与对照相比差异有统计学意义(P<0.01),且具有剂量依赖关系。结论 PPARγ被配体TGZ激活后抑制绒癌JEG-3细胞浸润能力,这为PPARγ配体在绒癌治疗中可能的临床应用提供理论和实验基础。
Objective To investigate the effect of PPARγ and Troglitazone (TGZ) on the invasive ability of choriocarcinoma JEG-3 cells. Methods The expression of PPARγ in JEG-3 cell line was detected by immunofluorescence cytochemistry. The invasive ability of choriocarcinoma cell line TGZ was detected by Transwell invasion system. Results The expression of PPARγ protein in choriocarcinoma JEG-3 cell line was significantly lower than that in control group (P <0.01), and there was a dose-dependent relationship. Conclusion PPARγ is inhibited by Ligand TGZ and inhibits the invasive ability of JEG-3 cells in choriocarcinoma, which provides a theoretical and experimental basis for the possible clinical application of PPARγ ligand in the treatment of choriocarcinoma.