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目的:探讨绿色荧光蛋白(GFP)基因转染本身是否可以改变细胞复合材料的生物学特性,从而影响材料生物相容性的正确评价。方法:将增强型绿色荧光蛋白(EGFP)转基因技术成功标记人源骨髓间充质干细胞(MSCs)诱导分化的成骨样细胞,再与人源生物衍生骨复合培养;同时设立未转染EGFP基因的骨髓MSCs诱导分化的成骨样细胞直接与人源生物衍生骨复合培养作为对照,通过倒置显微镜、荧光显微镜、扫描电镜对比观察。结果:发现转染EGFP基因的MSCs诱导分化的成骨样细胞能够成功地复合于人源生物衍生骨上,且生长状态较好。与未转染组细胞复合材料的情况相比,转染组与其没有明显差异。结论:EGFP基因转染技术没有对MSCs诱导分化的成骨样细胞复合衍生骨的能力以及增殖生长活性造成明显损害,从而不会影响对于材料生物相容性的正确评价。
Objective: To investigate whether green fluorescent protein (GFP) gene transfection itself can change the biological characteristics of cellular composites, thus affecting the correct evaluation of biocompatibility of materials. Methods: The osteoblast-like cells induced by human bone marrow mesenchymal stem cells (MSCs) were successfully labeled with enhanced green fluorescent protein (EGFP) transfection technique and then cultured with human derived bone. The untransfected EGFP gene Of bone marrow-derived MSCs differentiated into osteoblast-like cells directly with human-derived bio-derived bone composite culture as a control, by inverted microscope, fluorescence microscopy, scanning electron microscopy comparison. RESULTS: Osteoblast-like cells induced by MSCs transfected with EGFP gene were found to be successfully complexed with human-derived bone and grew well. Compared with the untransfected cells, the transfected group showed no significant difference. CONCLUSION: The EGFP gene transfection technique does not significantly impair the ability of MSCs to differentiate into osteoblast-like cells and their proliferative growth activity, so as not to affect the correct evaluation of the biocompatibility of the material.