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目的探讨颌下腺(SMG)导管细胞的体外培养方法。方法将SMG导管细胞进行原代和传代培养,观察培养细胞的形态结构,了解细胞的生长特性。同时经噻唑蓝(MTT)比色法检测细胞活力。结果导管细胞生长期间单层生长,呈多角形上皮细胞,可传代生长3～4代,存活3～4周。其中培养72h后,原代、第2代细胞活性与第4代相比较差异有统计学意义(P<0.05)。结论体外培养的原代及第2代细胞具有较高的增殖能力,可用于进一步研究。
Objective To investigate the in vitro culture method of submandibular gland (SMG) ductal cells. Methods Primary and subcultured SMG ductal cells were cultured and the morphology of cultured cells was observed to understand the growth characteristics of the cells. At the same time, cell viability was detected by MTT assay. Results The monolayer growth of ductal cells was polygonal epithelial cells, which could be passaged for 3-4 passages and survived for 3-4 weeks. After cultured for 72h, the viability of primary and second generation cells was significantly different from that of the fourth generation (P <0.05). Conclusion The primary and second generation cells cultured in vitro have high proliferative capacity and can be used for further study.