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目的 探讨沙土鼠短暂性脑缺血后灰质区少突胶质细胞选择性易损性及亚低温的影响。方法 阻断沙土鼠双侧颈总动脉 15分钟造成前脑缺血模型。实验动物被随机分为假手术组、缺血再灌流组、亚低温 (32 .5± 0 .5℃ )治疗组。采用细胞特异性抗原转铁蛋白 (TF)免疫组化法标记少突胶质细胞。结果 缺血后再灌注 1～ 2天 ,皮层区 TF阳性的少突胶质细胞密度显著下调 (P<0 .0 1) ,随后逐渐增加 ,于缺血后再灌注第14天达高峰。在亚低温处理的动物 ,皮层区 TF阳性少突胶质细胞密度早期下调和晚期上调均受到显著抑制(P<0 .0 1)。结论 灰质区少突胶质细胞具有选择性缺血易损性。亚低温治疗可防治缺血性少突胶质细胞损伤 ,并有可能用来防止缺血后脱髓鞘变性
Objective To investigate the selective vulnerability of oligodendrocytes and the effect of mild hypothermia in gerbils after transient cerebral ischemia. Methods Blocking bilateral common carotid arteries in gerbils for 15 minutes resulted in a model of forebrain ischemia. The experimental animals were randomly divided into sham operation group, ischemia-reperfusion group and mild hypothermia (32.5 ± 0.5 ℃) treatment group. Oligodendrocytes were labeled with the cell-specific antigen transferrin (TF) immunohistochemistry. Results The density of TF-positive oligodendrocytes in cortical area was significantly decreased (P <0.01) at 1 ~ 2 days after ischemia and then increased gradually, reaching the peak on the 14th day after ischemia and reperfusion. In hypothermia-treated animals, the early and late up-regulation of TF-positive oligodendrocyte density in cortical areas was significantly suppressed (P <0.01). Conclusions Gray matter oligodendrocytes have selective ischemic vulnerability. Mild hypothermia treatment can prevent ischemic oligodendrocyte injury, and may be used to prevent post-ischemic demyelinating degeneration