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水稻种子发芽率是在适宜的温度(30～15℃)和湿度条件下,经一定的时间(7～30天)后,发芽的种子数占供试种子数的百分比。发芽率的高低是衡量种子质量和实用价值的主要依据。用常规方法测定发芽率,需要时间较长。现介绍一种快速测定法—氯化三苯四氮唑(TTC)法,能在10～24小时内得出结果。众所周知,凡是有生活力的种子,胚部在呼吸过程中都有氧化还原反应,而无生命力的种子,胚则无氧化还原反应。此反应的存在,使得原来无色的氯化三苯四氮唑,还原成红色不溶性的三苯基甲(月朁)(TTF)。需用的仪器和药品:镊子、刀片、烧杯、培养皿、恒温箱。药品:称0.5%氯化三苯四氮唑溶液
The germination rate of rice seeds is the percentage of seeds germinated under certain temperature (30 ~ 15 ℃) and humidity after a certain time (7 ~ 30 days). The germination rate is the main basis for measuring seed quality and practical value. Determination of the germination rate using conventional methods, takes a long time. Now introduced a rapid determination of - trichloro-tetrazolium chloride (TTC) method, in 10 to 24 hours to reach the result. As we all know, all living seeds, embryos in the respiratory process have redox reactions, and non-viable seeds, the embryo is no redox reaction. The presence of this reaction allows the original colorless triphenyltetrazolium chloride to be reduced to red insoluble triphenylmethane (TTF). Need to use instruments and medicines: tweezers, blades, beakers, petri dishes, incubators. Drugs: Weigh 0.5% triphenyltetrazolium chloride solution