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目的：研究女性激素、增殖细胞核抗原（ＰＣＮＡ）、原癌基因ｃ－ｅｒｂＢ－２蛋白在子宫内膜增生过长和子宫内膜腺癌发病中的作用及对癌细胞分化的影响，为临床内分泌治疗提供理论依据。方法：采用免疫组织化学ＳＰ法，对手术切除和诊刮的３７例子宫内膜腺癌和２０例子宫内膜增生过长标本进行ＥＲ、ＰＲ、ＰＣＮＡ和ｃ－ｅｒｂＢ－２含量检测。并结合组织学类型和分化类型进行分析。结果：内膜腺癌组织中ＥＲ、ＰＲ的含量低于增生过长内膜组织（Ｐ＜０．０１），与肿瘤的组织学类型和分化有关。ＰＣＮＡ和ｃ－ｅｒｂＢ－２在内膜腺癌中的含量显著高于增生过长内膜组织（Ｐ＜０．０１），与分化程度呈负相关。与ＥＲ、ＰＲ的含量无明显相关性。结论：ＥＲ在子宫内膜增生过长和内膜腺癌的发病中起主要作用，ＰＣＮＡ和ｃ－ｅｒｂＢ－２的过表达可能与细胞的异常生长和分化有关。
Objective: To study the role of female hormones, proliferating cell nuclear antigen (PCNA) and proto-oncogene c-erbB-2 in endometrial hyperplasia and endometrial adenocarcinoma and its effect on differentiation of cancer cells. Treatment provides a theoretical basis. METHODS: ER, PR, PCNA and c-erbB-2 levels were detected in 37 specimens of endometrial adenocarcinoma and 20 specimens of endometrial hyperplasia by immunohistochemical SP method. And combined with histological type and differentiation type analysis. RESULTS: The contents of ER and PR in endometrial adenocarcinoma were lower than those in hyperplastic and intimal tissues (P<0.01), which was related to histological type and differentiation. The contents of PCNA and c-erbB-2 in the adenocarcinoma of the endometrium were significantly higher than those in the hyperplasia and intimal tissue (P<0.01), and negatively correlated with the degree of differentiation. There was no significant correlation with the contents of ER and PR. Conclusion: ER plays an important role in the development of endometrial hyperplasia and endometrial adenocarcinoma. The overexpression of PCNA and c-erbB-2 may be related to the abnormal growth and differentiation of cells.