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目的:探讨结直肠癌患者手术前后循环DNA水平的变化及其临床意义。方法:检测42例结直肠癌患者术前、术后3,14,30 d循环DNA水平,并检测同期14例健康体检者循环DNA水平作为对照。结果:结直肠癌患者术前循环DNA水平明显高于健康人[(92.25±46.88)ng/mL vs.(22.14±16.16)ng/mL](P<0.01);结直肠癌术前循环DNA水平在不同年龄、性别、肿瘤部位分组间差异无统计学意义(P=0.293,P=0.244,P=0.135)。结直肠癌患者术后3 d循环DNA水平达(114.95±62.41)ng/mL,较术前明显升高(P<0.01),但在术后14,30 d分别为(38.50±37.71)ng/mL与(31.69±41.48)ng/mL,明显低于术前(均P<0.01)。结论:循环DNA水平在结直肠癌患者中升高,且术后早期一过性进一步升高,随后明显下降。循环DNA水平有望成为结直肠癌术后监测肿瘤状态和评价疗效的标志物。
Objective: To investigate the changes of circulating DNA levels in patients with colorectal cancer before and after surgery and its clinical significance. Methods: DNA levels of 42 patients with colorectal cancer before operation and 3, 3, 30 and 30 days after operation were detected, and the level of circulating DNA in 14 healthy subjects was detected as control. Results: The preoperative circulating DNA level in patients with colorectal cancer was significantly higher than that in healthy people [(92.25 ± 46.88) ng / mL vs. (22.14 ± 16.16) ng / mL] (P <0.01) There was no significant difference among different age, sex and tumor sites (P = 0.293, P = 0.244, P = 0.135). The level of circulating DNA in patients with colorectal cancer was (114.95 ± 62.41) ng / mL 3 days after operation, which was significantly higher than that before operation (P <0.01), but it was (38.50 ± 37.71) ng / mL and (31.69 ± 41.48) ng / mL, respectively, which were significantly lower than those before operation (all P <0.01). Conclusion: The level of circulating DNA in patients with colorectal cancer increased, and the early postoperative transient increased further, and then decreased significantly. Circulating DNA levels are expected to be markers of colorectal cancer postoperative monitoring of tumor status and evaluation of efficacy.