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目的 :探讨喉粘膜上皮不典型增生定性诊断和早期喉癌诊断的方法。方法 :应用 p53基因PCR- SSCP分析、P53蛋白和 型胶原纤维蛋白免疫组化方法、流式细胞技术 (测细胞周期 )和核仁组成区嗜银蛋白 (Ag NORs)染色对 34例不典型增生上皮 (DS)、1 9例喉癌和 1 7例声带息肉的石蜡包埋组织进行研究。结果 :在 34例 DS中有 1 1例上述指标均为阳性 ,其中有 7例与病理诊断为癌相符合 ,有 4例病理诊断为良性 ;有 5例上述指标均为阴性 ,其中 2例病理诊断为癌 ,1例为良性 ,另外 2例无随访结果。结论 :只有将形态学指标和生物学指标联合应用 ,才能科学准确地对癌前病变进行定性诊断 ,对早期喉癌进行正确诊断
Objective: To explore the method of qualitative diagnosis of atypical hyperplasia of the laryngeal mucosa and diagnosis of early laryngeal cancer. Methods: Polymerase chain reaction was performed on 34 cases of atypical hyperplasia by PCR-SSCP analysis of p53 gene, immunohistochemistry of P53 protein and collagen fiber, flow cytometry (FCM) and Ag NORs staining. Epithelial (DS), 19 laryngeal carcinomas and 17 paraffin-embedded tissues of vocal cord polyps. Results: Of the 34 DS cases, all of the above indexes were positive, of which 7 were coincident with the pathological diagnosis of cancer and 4 were pathologically diagnosed as benign; 5 of them were negative, of which 2 were pathological One was diagnosed with cancer, one was benign, and the other two had no follow-up results. Conclusion: Only by combining the morphological and biological indicators can we diagnose precancerous lesions scientifically and accurately and diagnose early laryngeal cancer correctly