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目的 研究喉癌中 p16、CDK4蛋白的表达情况及其与临床病理特点的关系。方法 以抗 p16、抗 CDK4抗体为标记物 ,采用 SP(Streptavidin Peroxidase)免疫组织化学方法对 2 8例喉癌及 13例声带息肉石蜡标本进行分析。结果 p16在喉癌和声带息肉中的阳性率分别为 6 0 .71%和10 0 % (P<0 .0 1) ;CDK4在喉癌和声带息肉中的阳性率分别为 6 4.2 9%和 2 3.0 8% (P<0 .0 5 ) ;p16在喉癌 级组和 级以上组中的阳性率分别为 87.5 %和 2 5 % (P<0 .0 1)。CDK4在喉癌 级组和 级以上组中的阳性率分别为 5 6 .2 5%和 75 % (P>0 .0 5 )。结论 p16及 CDK4蛋白在喉癌的发生中可能起重要作用 ,p16的表达与喉癌病理分级相关
Objective To study the expression of p16 and CDK4 in laryngeal carcinoma and its relationship with clinicopathological features. Methods Anti-p16 and anti-CDK4 antibodies were used as markers to detect 28 cases of laryngeal cancer and 13 cases of vocal cord polyp paraffin specimens by Streptavidin Peroxidase (SP) immunohistochemistry. Results The positive rates of p16 in laryngeal carcinoma and vocal cord polyps were 60.71% and 100% respectively (P <0.01). The positive rates of CDK4 in laryngeal carcinoma and vocal cord polyp were 62.29% and 2 3.08% (P <0.05). The positive rate of p16 was 87.5% and 25% (P <0.01) in the laryngeal squamous cell carcinoma group and the above-grade group, respectively. The positive rates of CDK4 in the laryngeal cancer group and above were respectively 56.2% and 75% (P> 0.05). Conclusion The expressions of p16 and CDK4 may play an important role in the carcinogenesis of laryngeal carcinoma. The expression of p16 is correlated with the pathological grade of laryngeal carcinoma