论文部分内容阅读
目的构建大鼠孕期营养性铁缺乏模型,探讨母鼠缺铁贫血对仔鼠生长发育的影响。方法将6周龄健康Wistar雌性大鼠随机分为2组,实验组即低铁组喂饲低铁饲料,对照组喂饲富铁饲料。4周后与健康成年Wistar雄鼠同笼交配,同笼前检测体重、血红蛋白(Hb)、红细胞分布宽度(RDW-CV)、铁蛋白等值(Fn),待雌鼠产仔观测雌鼠受孕率、雌鼠产仔活产率、活胎数及幼仔断乳日存活率及其生长状况。结果干预4周后(同笼前)低铁组血红蛋白(Hb)为(123.07±12.18)g/L,体重等值均低于对照组(P<0.05);红细胞分布宽度(RDW)为(26.53±5.31)%,游离红细胞原卟啉(FEP)为1.97±0.41,均高于对照组(P<0.05)。对照组雌鼠与低铁组雌鼠受孕率相同,均为83.3%;对照组仔鼠与低铁组仔鼠相比,活胎率和存活率及其生长速度差异均有统计学意义(P<0.05)。结论母鼠缺铁性贫血可影响仔鼠生长发育。
Objective To construct a model of nutritional iron deficiency during pregnancy in rats and investigate the effects of iron deficiency anemia in female rats on the growth and development of offspring. Methods Six-week-old healthy Wistar female rats were randomly divided into two groups. In the experimental group, rats in the low-iron group were fed with low-iron diet, while those in the control group were fed with iron-rich diet. Four weeks later, males were mated with healthy adult Wistar male rats. Body weights, hemoglobin (Hb), RDW-CV and Fn were measured before cage formation. Rate, live birth rate of female rats, number of live fetuses and weaning day survival rates and their growth status. Results Hemoglobin (Hb) in low iron group was (123.07 ± 12.18) g / L after 4 weeks of intervention (equal to cage), body weight was lower than that in control group (P <0.05) ± 5.31)%, while the free erythrocyte protoporphyrin (FEP) was 1.97 ± 0.41, both of which were higher than those of the control group (P <0.05). The female and low-iron female rats in the control group had the same conception rate of 83.3%, and there were significant differences in the live birth rate, the survival rate and the growth rate between the control group and the low iron group (P <0.05). Conclusion Femal iron deficiency anemia can affect the growth and development of offspring.