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目的 研究糖尿病大鼠角膜上皮创伤愈合过程中胰岛素样生长因子-1（insulin-like growth factor-1, IGF-1）及胰岛素样生长因子1受体(insulin-like growth factor-1 receptor, IGF-1R)的表达，从分子水平探讨糖尿病角膜上皮创伤愈合延迟的可能机制。方法 链脲霉素诱导糖尿病大鼠模型，造模后每周同一时间点监测体质量和空腹血糖变化。制作大鼠角膜上皮创伤模型，于创伤后不同时间点，评价角膜上皮愈合速率；实时定量PCR及免疫荧光检测角膜上皮中IGF-1、IGF-1R的表达。结果 糖尿病组体质量低于正常组，空腹血糖浓度高于正常组。与正常组相比，糖尿病组角膜上皮愈合速率慢。实时定量PCR及免疫荧光检测显示，新愈合的糖尿病组大鼠角膜上皮中IGF-1、IGF-1R的mRNA、蛋白表达低于正常组。结论 糖尿病大鼠角膜上皮创伤愈合延迟，IGF-1、IGF-1R表达下调可能是导致愈合延迟的原因之一。
Objective To investigate the effects of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor-1 receptor (IGF) on the process of corneal epithelial wound healing in diabetic rats -1R) expression at the molecular level to explore the possible mechanism of delayed wound healing of diabetic corneal epithelium. Methods Streptozotocin induced diabetic rat model, body weight and fasting blood glucose levels were monitored at the same time every week. The rat corneal epithelial wound model was established. The healing rate of corneal epithelium was evaluated at different time points after trauma. The expression of IGF-1 and IGF-1R in corneal epithelium was detected by real-time quantitative PCR and immunofluorescence. Results The body weight of diabetic group was lower than that of normal group, and the fasting blood glucose concentration was higher than that of normal group. Compared with the normal group, the corneal epithelial healing rate in diabetic group was slow. Real-time quantitative PCR and immunofluorescence assay showed that the mRNA and protein expressions of IGF-1 and IGF-1R in corneal epithelium of newly-healed diabetic rats were lower than those in normal rats. Conclusion The wound healing of corneal epithelium in diabetic rats is delayed, and the down-regulation of IGF-1 and IGF-1R may be one of the reasons leading to delayed healing.