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一、菠萝根腐病病原菌的分离将感病枯萎的菠萝根切断放入烧杯里,按每10条根20毫升兑入消毒水,再将健全的菠萝叶(从心叶至第10枚叶)、胡萝卜和马铃薯切成宽5毫米的长条,放入上述烧杯里,在28℃下静置4—5天后取出,分别放在PSA培养基中进行分离培养。结果为,用菠萝叶培养的,疫霉菌属的致病率为46.9%,其它菌(主要是木霉属和镰刀菌属)的致病率为17.73%;用胡萝卜培养的,疫霉属的致病率为
First, the separation of pineapple root rot pathogens Sear of withered pineapple root cut into the beaker, according to 10 ml of root into the disinfectant 20 ml, and then a healthy pineapple leaf (from the heart to the first 10 leaves) , Carrot and potato cut into 5 mm wide strips, placed in the beaker, at 28 ° C for 4-5 days after taking out, were placed in the PSA culture medium for separation and culture. As a result, the prevalence of Phytophthora was 46.9% in pineapple leaves and that of other bacteria (mainly Trichoderma and Fusarium) was 17.73%. Phytophthora The incidence rate is