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以玉米黄早四自交系为材料，采用生物素标记的染色体原位杂交对与大斑病抗性基因Ｈｔｎ１紧密连锁的两侧２个ＲＦＬＰ标记ＵＭＣ８４和ＵＭＣ３０进行了定位。结果表明：ＵＭＣ８４和ＵＭＣ３０探针都同时与第８和第６染色体杂交，平均信号检出率为１４．７％。ＵＭＣ８４和ＵＭＣ３０在第８号染色体长臂上杂交信号与着丝粒的百分距离分别为３８．２６±１．９７和３７．９２±３．４８。在第６号染色体长臂上杂交信号与着丝粒的百分距离分别为３３．５８±３．２８和３４．９６±２．１３。这些结果说明ＵＭＣ８４和ＵＭＣ３０之间的物理距离相距很近，以此推断ＵＭＣ８４或ＵＭＣ３０的位置所在区域应该是Ｈｔｎ１所处的区域。
Two inbred lines (Zea mays L.) inbred lines were used as biotin-labeled chromosomes to locate two RFLP markers, UMC84 and UMC30, which were closely linked to Htn1. The results showed that both UMC84 and UMC30 probes hybridized with chromosome 8 and 6 simultaneously, and the average signal detection rate was 14.7%. The percentages of centromeres and centromeres between the hybridization signals of UMC84 and UMC30 on the long arm of chromosome 8 were 38.26 ± 1.97 and 37.92 ± 3.48, respectively. The percentage of hybridization signals to centromeres on the long arm of chromosome 6 were 33.58 ± 3.28 and 34.96 ± 2.13, respectively. These results indicate that the physical distance between UMC84 and UMC30 is very close to infer that the area where UMC84 or UMC30 is located should be the area where Htn1 is located.