Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective swe

• 作 者: Dongyun XIN Yangyang BAI Yi BI Libang HE Yuxin KANG Chuanying PAN Haijing ZHU Hong CHEN Lei QU Xianyong LAN
• 机 构: Lab of Animal Genome and Gene Function,College of Animal Science and Technology,Shaanxi Key Laborato
• 出 处: 浙江大学学报（英文版）（B辑：生物医学和生物技术）
• 发表日期: 2021年9期
• 关键词: Goat Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) Litter size I

Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2, also called IMP2) plays an essential role in the development and maturation of germ cells and embryos and is a candidate gene for goat litter size, based on a previous genome-wide selective sweep analysis. In this study, the mRNA expression level of IGF2BP2 was found to be significantly higher in a single-lamb group than in a multi-lamb group. Insertions/deletions (indels) within the goat IGF2BP2 gene, including P4-Ins-13bp and P5-Del-12bp, were verified in 918 Shaanbei White Cashmere (SBWC) female goats. The minor allelic frequencies (MAFs) of P4-Ins-13bp and P5-Del-12bp loci were 0.349 and 0.295, respectively. Analysis using the Chi-square (χ2) test showed that the genotype (χ2=14.479, P=0.006) distribution of P4-Ins-13bp was significantly different between the single-lamb and multi-lamb groups. Correlation analysis demonstrated that P4-Ins-13bp was significantly associated with goat litter size (P=0.022), and individual goats with the homozygous deletion/deletion (DD) genotype produced more litters than other goats. Therefore, considered as a potential molecular marker significantly related to lambing traits, the P4-Ins-13bp mutation of the goat IGF2BP2 gene can be used in goat breeding with practical molecular marker-assisted selection (MAS) to optimize female reproduction and improve economic efficiency in the goat industry.