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多倍体育种我们于1978年,开始对丝瓜进行多倍体育种,选用长沙肉丝瓜(图左)为原材料。每组20株幼苗,温度19—25℃,药液浓度为1000ppm、2000ppm、3000ppm的秋水仙碱。在幼苗子叶半展开时,用脱脂小棉球放于胚芽处,滴入秋水仙药液,每日约3次,以保持棉球湿润,达到持续作用的目的。试验结果表明,药液浓度为2000ppm,处理时间为48小时,温度为25℃左右时为最适宜,诱导率为25％。浓度过大,如3000ppm造成胚芽细胞中毒死亡。浓度不够,如1000ppm,不能形成多倍体。浓度适当,但处理时间过长,造成染色体加倍后再加倍,往往
Polyploidy We started in 1978, polyploidy loofah species, the choice of Changsha shredded loofah (left) as raw material. Each group of 20 seedlings, temperature 19-25 ℃, liquid concentration of 1000ppm, 2000ppm, 3000ppm colchicine. Semi-expanded seedlings in the cotyledons, with cotton wool ball placed in the germ Department, dripping into the immortality liquid, about 3 times a day to keep the cotton ball moist, to achieve the purpose of continued action. The test results show that the solution concentration of 2000ppm, the treatment time of 48 hours, the temperature is about 25 ℃ when the most appropriate, the induction rate of 25%. Concentration is too large, such as 3000ppm cause germ cell poisoning death. Concentration is not enough, such as 1000ppm, can not form polyploid. Concentration appropriate, but the processing time is too long, resulting in doubling the chromosome and then doubled, often