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随着单克隆抗体技术的发展,目前出现了许多抗人T细胞表面抗原的单克隆抗体,对这些表面抗原的检测,国内学者大多利用间接免疫荧光技术。我室用异硫氰酸荧光素(FITC)成功地标记了多种抗人体T细胞亚群的单克隆抗体,这对研究T细胞亚群在感染性疾病、自身免疫性疾病、免疫缺陷性疾病中的变化,以及检测免疫抑制治疗等提供了有力工具。 OKT系列单克隆抗体为从美国ATCC公司引进的细胞株,由本室制备。抗-CD3(OKT3)、抗-CD4(OKT4)、抗-CD8(OKT8)、抗-CD2(OKT11)、抗-CD1(OKT6)、抗-CD38(OKT10)、抗-CD11b(OKM1),还有抗HLA—Ⅰ类抗原及HLA—Ⅱ类抗原的单抗。各种单抗腹水用硫酸铵粗提丙球和经SPA-
With the development of monoclonal antibody technology, there are many monoclonal antibodies against human T cell surface antigen. For the detection of these surface antigens, most scholars in China use indirect immunofluorescence technique. Our laboratory successfully labeled a variety of anti-human T cell subsets monoclonal antibodies with fluorescein isothiocyanate (FITC), which is of great importance for the study of T cell subsets in infectious diseases, autoimmune diseases, immunodeficiency diseases In the changes, as well as the detection of immunosuppressive therapy provides a powerful tool. OKT series of monoclonal antibodies from the United States ATCC company introduced cell lines, the room prepared. Anti-CD3, OKT4, OKT8, OKT11, OKT6, OKT10 and OKM1 also Anti-HLA class I antigens and HLA class II antigens monoclonal antibody. A variety of monoclonal antibody ascites with crude ammonium sulfate balls and SPA-