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合成钯-四氧化三铁纳米粒子(Pd-Fe3O4NPs)并制备了乙酰胆碱酯酶(AChE)电流型生物传感器用于检测有机磷杀虫剂。Pd-Fe3O4NPs在电极与乙酰胆碱酯酶之间提供了一个具有较高电子传导性、较好生物相容性和较强催化活性的微环境,有效地提高了乙酰胆碱酯酶的生物活性和有机磷杀虫剂对乙酰胆碱酯酶的抑制作用。以对硫磷为有机磷杀虫剂模型分子,乙酰胆碱酶的抑制率与对硫磷浓度在5×10-12～1×10-9mol/L之间呈现良好的线性关系,检测限为2.5×10-12mol/L。
Synthesis of palladium-iron tetroxide nanoparticles (Pd-Fe3O4NPs) and prepared acetylcholinesterase (AChE) current-based biosensors for the detection of organophosphate insecticides. Pd-Fe3O4NPs provide a microenvironment with high electron conductivity, good biocompatibility and strong catalytic activity between the electrode and acetylcholinesterase, effectively increasing the biological activity of acetylcholinesterase and the efficiency of organophosphate killing Inhibitory effect of insecticides on acetylcholinesterase. With parathion as an organophosphorus pesticide model molecule, the inhibitory rate of acetylcholinease and paracetamol concentration between 5 × 10-12 ~ 1 × 10-9mol / L showed a good linear relationship between the detection limit of 2.5 × 10-12mol / L.