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同步化协同诱导可以稳定提高曼地亚红豆杉细胞培养物中紫杉醇含量。细胞培养周期第8d的低温同步化处理可促使细胞达到最高同步化率(20.4%),而茉莉酸甲酯(MJ)的协同诱导可提高紫杉醇产量,使紫杉醇产量最高值达到54.7mg·L-1。在细胞生长周期第8d,未经低温同步化的红豆杉细胞中的关键酶基因DXR、HMGR、GGPPS和DBAT的表达量在MJ诱导24h后均迅速下降,但在低温同步化的细胞中紫杉醇表达量下降缓慢,60h后仍维持较高的水平。低温同步化和MJ协同诱导的红豆杉细胞中,紫杉醇合成关键酶基因高效稳定的表达可能是引起紫杉醇产量稳定提高的原因之一。
Synchronization synergistic induction can steadily increase paclitaxel content in cell culture of Taxus media. Synergistic hypothermia was observed at the 8th day of cell culture, which led to the highest synchronization rate (20.4%). Synergistic induction of methyl jasmonate (MJ) increased the paclitaxel production and reached the highest value of 54.7 mg · L- 1. The expression of DXR, HMGR, GGPPS and DBAT, the key enzyme genes in Taxus cuspidata cells without hypothermia, decreased rapidly at 24th day after induction of MJ, but paclitaxel expression in cells synchronized with hypothermia Slow decline in volume, still maintain a high level after 60h. One of the reasons for the stable increase of paclitaxel production may be that the expression of paclitaxel key enzyme gene in Taxus cuspidata cells co-induced by hypothermia and MJ synergism.