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目的探索浙江省畲汉两族人群ACE基因多态性分布情况及其与原发性高血压的关系。方法采用聚合酶链反应(PCR)检测215例畲汉两族原发性高血压(EH)及正常人群血中ACE基因16内含子I/D多态标记,分析计算各基因型(DD型、ID型、Ⅱ型)及等位基因频率,运用Logistic多元回归分析ACE基因型与原发性高血压的关系。结果浙江畲族EH组与对照组DD、ID、Ⅱ基因型频率分别为39.7%、32.8%、27.6%及18.8%、45.8%、35.4%,D和I等位基因分别为56.0%、44.0%及41.7%、58.3%。汉族EH组与对照组DD、ID、Ⅱ基因型频率分别40.7%、31.5%、27.8%及21.8%、40.0%、38.2%,D和I等位基因分别为56.5%、43.5%及41.8%、58.2%。Logistic回归显示DD基因型为原发性高血压危险因素(OR=2.746,P<0.05)。结论畲汉两族之间ACE基因多态性构成没有差异,两族中带有DD基因型及D等位基因的个体易患原发性高血压。
Objective To explore the distribution of ACE gene polymorphism and its relationship with essential hypertension in She and Han populations in Zhejiang Province. Methods Polymerase chain reaction (PCR) was used to detect the polymorphism of ACE gene 16 intron I / D polymorphism in blood of 215 cases of EH in Han and Shensi families and normal controls. The genotypes of DD , ID type, type Ⅱ) and allele frequency, using Logistic multiple regression analysis of ACE genotype and essential hypertension. Results The frequencies of DD, ID, and II genotypes in Zhejiang EH and control groups were 39.7%, 32.8%, 27.6% and 18.8%, 45.8% and 35.4%, respectively. The frequencies of D and I alleles were 56.0% and 44.0% 41.7%, 58.3%. The frequencies of DD, ID, and II genotypes in Han Han and Han controls were 40.7%, 31.5%, 27.8% and 21.8%, 40.0% and 38.2%, respectively. The frequencies of D and I alleles were 56.5%, 43.5% and 41.8% 58.2%. Logistic regression showed that the DD genotype was a risk factor for essential hypertension (OR = 2.746, P <0.05). Conclusion There is no difference in ACE gene polymorphism between Shek and Han ethnic groups. Individuals with DD genotype and D allele in both groups are predisposed to essential hypertension.