氯菊酯和氯氰菊酯对大鼠脑突触体Ca~(2+)-和Ca~(2+),Mg~(2+)-ATP酶活性的抑制

• 作 者: 唐促跃 马谭庚 刘毓谷
• 机 构: 同济医科大学环境毒理研究室,同济医科大学环境毒理研究室,同济医科大学环境毒理研究室 武汉 430030 华西医科大学公共卫生学院 610044,武汉 430030,武汉 430030
• 出 处: 中国药理学与毒理学杂志
• 发表日期: 1990年01期
• 关键词: ATP Ca Mg 氯菊酯 突触体 Ca~(2+)-ATP酶 大鼠脑突触体 鼠脑 酶活性 非竞争性抑制

氯菊酯可明显抑制Ca~(2+)—ATP酶活性,其抑制作用是可逆的,底物动力学分析表明为非竞争性抑制,介质的酸度可影响其抑制效应;氯氰菊酯对该酶活性无明显影响.氯菊酯和氯氰菊酯均能可逆性地抑制Ca~(2+),Mg~(2+)—ATP酶活性,但氯菊酯为反竞争性抑制.且有一定的pH依赖性,而氯氰菊酯为非竞争性抑制,其抑制率基本不受pH的影响,表明这两种杀虫剂在Ca~(2+) ,Mg~(2+)—ATP酶上有不同的结合位点.

Permethrin inhibited the activity of Ca ~ (2 +) - ATPase and its inhibition was reversible. The substrate kinetic analysis showed that it was a noncompetitive inhibitor. The acidity of the medium could affect its inhibitory effect. Cypermethrin could inhibit the enzyme activity No significant effect.Permethrin and cypermethrin can reversibly inhibit the activity of Ca ~ (2 +), Mg ~ (2 +) - ATPase, but permethrin is anticompetitively inhibited and has certain pH dependence , While cypermethrin was a non-competitive inhibitor. The inhibition rate was not affected by pH, indicating that the two insecticides had different binding sites on Ca 2+ and Mg 2+ -ATPase .