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A comparative study on chloroplast ultrastructure and light harvesting complex of photosystem Ⅱ (LHC Ⅱ) was conducted between a new rice mutant (W2555) and its wild type (WT). The chloroplasts of W2555 had less thylakoids and grana stacks compared with the wild type. There was no significant change in the composition of LHC Ⅱ polypeptide in W2555, while a decline had been noted in LHC Ⅱ content. Northern blot analysis with a specific cab gene probe showed no appreciable difference in the LHC Ⅱ mRNA level between the W2555 and its wild type. The precursors of chlorophyll synthesis, δ-aminolevulinic acid (ALA) and porphobilinogen (PBG) were over accumulated in W2555, but the other precursors were all decreased. These results indicated that the decreased level of LHC Ⅱ in the mutant W2555 was attributed to the change of cab gene transcription, but a blockage in chlorophyll biosynthesis due to the formation of uroporphyrinogen Ⅲ (Urogen Ⅲ).
A comparative study on chloroplast ultrastructure and light harvesting complex of photosystem II (LHC II) was conducted between a new rice mutant (W2555) and its wild type (WT). The chloroplasts of W2555 had less thylakoids and grana stacks compared with the wild type . There was no significant change in the composition of LHC II polypeptide in W2555, while a decline had been noted in LHCII content. Northern blot analysis with a specific cab gene probe showed no significant difference in the LHC II mRNA level between the W2555 and The wild type. The precursors of chlorophyll synthesis, δ-aminolevulinic acid (ALA) and porphobilinogen (PBG) were over accumulated in W2555, but the other precursors were all decreased. These results indicates that the decreased level of LHC Ⅱ in the mutant W2555 was attributed to the change of cab gene transcription, but a blockage in chlorophyll biosynthesis due to the formation of uroporphyrinogen III (Urogen III).