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目的:研究具斑芒毛苣苔抑制α-葡萄糖苷酶的活性成分。方法:用体外α-葡萄糖苷酶抑制模型筛选;采用各种色谱法对高活性部位分离,运用多种波谱技术鉴定结构。结果:从具斑芒毛苣苔分离得到7个化合物,分别为羽扇豆醇(1),豆甾醇(2),熊果酸(3),豆甾-5,22(E)-二烯-3β-醇(4)和β-胡萝卜苷(5),3-hydroxy-12-taraxasten-28-oic-acid(6),齐墩果酸(7)。化合物1(IC50 25.41 mg.L-1),3(IC50 4.42 mg.L-1),4(IC50 11.50 mg.L-1),6(IC50 14.17 mg.L-1)和7(IC50 2.88mg.L-1)的体外抑制α-葡萄糖苷酶活性高于阳性对照药物acarbose(IC501 103.01 mg.L-1)。结论:化合物1～7为首次从该植物中分离得到,6为首次从该科中分离得到,7为首次从该属植物中分离得到。
OBJECTIVE: To study the inhibitory activity of α-glucosidase from C. przewalskii. Methods: The in vitro α-glucosidase inhibition model was screened. Various chromatographic methods were used to separate the highly active fractions and the structures were identified by various spectral techniques. Results: Seven compounds were isolated from the genus Cochinchinensis and were identified as lupine alcohol (1), stigmasterol (2), ursolic acid (3), stigmasterol-5,22 (E) 3β-ol (4) and β-daucosterol (5), 3-hydroxy-12-taraxasten-28-oic-acid (6) and oleanolic acid (7). Compound 1 (IC50 25.41 mg.L-1), 3 (IC50 4.42 mg.L-1), 4 (IC50 11.50 mg.L-1), 6 (IC50 14.17 mg.L-1) and 7 (IC50 2.88 mg .L-1) inhibited α-glucosidase activity in vitro more than the positive control drug acarbose (IC501 103.01 mg.L-1). CONCLUSION: Compounds 1-7 were isolated from this plant for the first time, 6 was isolated from this family for the first time, and 7 was isolated from this genus for the first time.