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磁性纳米颗粒在纳米生物医学诊断和治疗研究领域是极具潜力的一种纳米材料.如何实现纳米颗粒在特定细胞或靶器官的高效率传输以及如何降低细胞毒性是目前纳米材料研究的重点内容.本文首先研究了12 nm的γ-Fe2O3磁性纳米颗粒进入细胞的三种不同途径:(1)纳米颗粒与肿瘤细胞共孵育后的内吞途径;(2)纳米颗粒与微气泡共混合后超声辐照传输途径;(3)纳米颗粒化学偶联到微气泡膜壳表面后超声辐照传输途径.其次,基于上述三种不同的纳米颗粒传输途径,对纳米颗粒引起的细胞氧化应激毒性进行了深入研究.结果表明,纳米颗粒与肿瘤细胞共孵育后的内吞途径使纳米颗粒通过溶酶体包裹进入细胞;通过超声微气泡辐照,纳米颗粒能够以更高效率通过非内吞途径直接传输进入细胞质而不被溶酶体包裹.不同传输途径导致纳米颗粒分别进入溶酶体和细胞质,造成对细胞内氧化应激水平、总抗氧化能力以及谷胱甘肽过氧化物酶活性的响应不同.综上研究表明,超声微气泡介导的磁性纳米颗粒传输能够成为一种高效无损的细胞纳米颗粒输运新方法,同时通过控制纳米颗粒进入细胞质降低了纳米颗粒的毒性,从而能够更广泛应用于纳米生物医学的应用研究.
Magnetic nanoparticles are promising nanomaterials in the field of nanomedicine diagnostics and therapeutics.How to achieve high efficiency nanoparticle transfer in specific cells or target organs and how to reduce cytotoxicity are the important contents of nanomaterials. In this paper, three different ways of entering 12 nm γ-Fe2O3 magnetic nanoparticles into cells were firstly studied: (1) Endocytic pathway after co-incubation of nanoparticles with tumor cells; (2) Ultrasound radiation after co-mixing nanoparticle and microbubbles (3) The nanoparticles were chemically coupled to the surface of the microbubble membrane after ultrasound irradiation transmission pathway.Secondly, based on the above three different routes of nanoparticle transmission, the oxidative stress induced by nanoparticles was studied The results showed that the endocytosis pathway after the nanoparticles were incubated with the tumor cells allowed the nanoparticles to enter the cells through lysosomes, and the nanoparticles could be directly transported by the non-endocytic pathway with higher efficiency by the ultrasonic microbubble irradiation Enter the cytoplasm without being surrounded by lysosomes.Different routes of transport cause the nanoparticles to enter lysosomes and cytoplasm respectively, Oxidative stress, total antioxidant capacity and glutathione peroxidase activity.In summary, it has been shown that ultrasound microbubble-mediated magnetic nanoparticle transfer can be an efficient and non-destructive cell nanoparticle transport The new method, at the same time through the control of nanoparticles into the cytoplasm to reduce the toxicity of nanoparticles, which can be more widely used in nano-biomedical applications.