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目的探讨阿苯达唑(albendazole,ABZ)对人耐顺铂卵巢癌SKOV-3/DDP细胞糖酵解的影响。方法通过溴化二甲噻唑二苯四氨唑(MTT)比色法检测ABZ对卵巢癌SKOV-3/DDP细胞体外增殖的影响,求得抑制率分别为0%、25%、50%、75%时所对应的ABZ的浓度,实验分为对照组、IC25组、IC50组和IC75组,每组给予相应浓度的ABZ处理,并且按照ABZ作用时间再将每组分为12、24、36 h亚组。按照分组,在设定的时间点,分别用比色法测定己糖激酶(hexokinase,HK)和丙酮酸激酶(pyruvate kinase,PK)活性,用酶标仪法测定乳酸脱氢酶(lactate dehydrogenase,LDH)的活性,用RT-PCR法测定Akt mRNA和Myc mRNA的表达。结果经过不同浓度的ABZ处理SKOV-3/DDP细胞发现,ABZ能抑制SKOV-3/DDP细胞体外生长(P<0.05),呈剂量依赖性,抑制率为0%所对应的ABZ浓度为0μmol/L,抑制率25%的浓度(IC25)为(1.43±0.21)μmol/L,抑制率50%的浓度(IC50)为(8.64±0.74)μmol/L,抑制率75%的浓度(IC75)为(52.48±3.99)μmol/L。与对照组相比,经过ABZ处理的SKOV-3/DDP细胞的HK、PK、LDH活性明显降低(P<0.05),且Akt基因和Myc基因的表达下调(P<0.05)。结论 ABZ能够抑制SKOV-3/DDP细胞糖酵解酶活性,并能下调糖酵解相关基因的表达。
Objective To investigate the effect of albendazole (ABZ) on the glycolytic activity of human cisplatin-resistant ovarian cancer cell line SKOV-3 / DDP. Methods The proliferation of SKOV-3 / DDP ovarian cancer cell line SKOV-3 / DDP was detected by methyl thiazolyl tetrazolium bromide (MTT) colorimetric assay. The inhibitory rates were 0%, 25%, 50%, 75% % Corresponding to the concentration of ABZ, the experiment is divided into control group, IC25 group, IC50 group and IC75 group, each group given the corresponding concentration of ABZ treatment, and according to the ABZ time and then each group was divided into 12,24,36 h Subgroup. According to the grouping, the activities of hexokinase (HK) and pyruvate kinase (PK) were determined by colorimetric method at the set time points respectively, and the activity of lactate dehydrogenase LDH) activity, Akt mRNA and Myc mRNA expression was determined by RT-PCR method. RESULTS: ABZ treatment inhibited the growth of SKOV-3 / DDP cells in vitro (P <0.05) in a dose-dependent manner. The ABZ concentration was 0μmol / (IC50) was (1.43 ± 0.21) μmol / L and IC50 was (8.64 ± 0.74) μmol / L and IC50 was 75% (IC50) was 25% (52.48 ± 3.99) μmol / L. Compared with the control group, the activities of HK, PK and LDH in SKOV-3 / DDP cells treated with ABZ were significantly decreased (P <0.05), and the expressions of Akt and Myc were down-regulated (P <0.05). Conclusion ABZ can inhibit the activity of glycolytic enzymes in SKOV-3 / DDP cells and down-regulate the expression of glycolysis-related genes.