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本文介绍一种用于疫苗的新佐剂,它不但能增强抗原的体液免疫性,而且还能增加细胞免疫应答。为了证实佐剂的安全性与免疫性,作者以卵蛋白为抗原,加入新佐剂作全面实验观察。将吐温-80、普鲁兰尼L121、角鲨烯与不同种类的胞壁酰二肽(MDP)混合制成不同佐剂,然后以1份佐剂与等量含有两剂量浓度的卵蛋白充分混匀。以豚鼠为实验动物,以磷酸盐缓冲液(PBS)代替佐剂作对照,于颈部皮下注射两针,用被动血凝试验测抗体;皮内注射观察迟发型变态反应;大腿肌肉注射观察局部红肿反应;测定血清肌酐磷激酶水平并观察早期反应症状、体重变化、注射部位的组织学变化和成淋巴细胞转化和白细胞介素2产生情况等。
This article describes a new adjuvant for vaccines that not only enhances the humoral immunity of an antigen, but also increases the cellular immune response. In order to confirm the safety and immunity of the adjuvant, the authors took ovalbumin as antigen and added new adjuvant for comprehensive experimental observation. Tween-80, pullulan L121, squalene and different types of muramyl dipeptide (MDP) were mixed to make different adjuvants, and then with 1 part adjuvant and equal amount contains two doses of egg protein Mix well Guinea pigs as experimental animals, phosphate buffered saline (PBS) instead of adjuvant as a control, subcutaneous injection in the neck two needles, with passive hemagglutination test antibody; intradermal injection to observe delayed-type hypersensitivity; thigh muscle injection to observe the local Swelling reaction; determination of serum creatinine kinase level and observe the early reaction symptoms, body weight changes, injection site histological changes and lymphoblast transformation and interleukin 2 production situation.