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目的：对１９ 例鼻咽部Ｂ细胞性恶性淋巴瘤与ＥＢ 病毒（ＥｐｓｔｅｉｎＢａｒｒ ｖｉｒｕｓ，ＥＢＶ） 感染的相关性进行研究。方法：利用免疫组织化学及原位杂交方法对ＥＢＶ 进行检测，并用免疫组化及原位杂交双染法标记肿瘤细胞，以鉴定ＥＢＶ阳性的细胞为Ｂ淋巴瘤细胞。结果：ＥＢＶ 编码的小ｍ ＲＮＡ探针（ＥＢＥＲ） 原位杂交显示，８ 例原发性鼻咽部Ｂ细胞性恶性淋巴瘤中３ 例绝大多数肿瘤细胞呈阳性表达，１ 例潜在性膜蛋白１（ｌａｔｅｎｔ ｍｅｍｂｒａｎｅ ｐｒｏｔｅｉｎ １，ＬＭＰ１）阳性。而１１ 例继发性鼻咽部Ｂ细胞性恶性淋巴瘤ＥＢＥＲ全部为阴性。全部病例进行了ＬＭＰ１ 检测，除１ 例原发者，全部阴性。利用ＥＢＥＲＩＳＨ（ＥＢＥＲ原位杂交） 和免疫组化ＣＤ 标记物进行双标记染色证实，ＥＢＥＲ 和ＬＭＰ１阳性细胞为ＣＤ２０ 阳性，ＣＤ４５ＲＯ 阴性。鼻咽部原发性Ｂ细胞性恶性淋巴瘤ＥＢＶ 表达４／８ ，而继发者为０／１１。结论：ＥＢＶ 与鼻咽部原发性Ｂ细胞恶性淋巴瘤有较高的相关性，而与继发性者无关。
Objective: To study the correlation between 19 cases of nasopharyngeal B-cell lymphoma and EpsteinBarr virus (EBV) infection. Methods: EBV was detected by immunohistochemistry and in situ hybridization. Tumor cells were labeled by immunohistochemistry and in situ hybridization to identify EBV-positive cells as B lymphoma cells. Results: EBV-encoded small m RNA probe (EBER) in situ hybridization showed that most of 8 cases of primary nasopharyngeal B-cell lymphoma were positive for tumor cells, and 1 case of latent membrane protein 1 (latent membrane protein 1, LMP 1) positive. Eleven cases of secondary nasopharyngeal B cell malignant lymphoma EBER were all negative. All cases were detected LMP 1, except for 1 case of primary, all negative. EBER ISH (EBER in situ hybridization) and immunohistochemical CD markers double labeling staining confirmed that EBER and LMP 1 positive cells CD20 positive, CD45RO negative. EBV expression in nasopharyngeal primary B-cell lymphoma was 4/8, with 0/11 in the secondary. Conclusion: EBV has a high correlation with nasopharyngeal primary B-cell malignant lymphoma, but not with secondary.