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采用双向电泳免疫印迹检测法，分析了２４ 例正常人及 ２４ 例Ⅳ型高脂血症患者血清 Ｈ Ｄ Ｌ亚类的相对百分含量。结果显示：与正常人比较，Ⅳ型高脂血症患者血清中小颗粒的前β１ Ｈ Ｄ Ｌ和 Ｈ Ｄ Ｌ３ａ含量显著增加（ Ｐ值分别＜ ０．００１ 和＜ ０．０５），而成熟的 Ｈ Ｄ Ｌ２ｂ含量显著减少（ Ｐ＜ ０．００１）。患者血清 Ｔ Ｇ 浓度与前β１ Ｈ Ｄ Ｌ及 Ｈ Ｄ Ｌ３ａ水平呈正相关（相关系数分别为 ０．５８２ 和 ０．６９２），而与 Ｈ Ｄ Ｌ２ｂ 及 Ｈ Ｄ Ｌ２ａ 水平呈显著负相关（相关系数分别为－ ０．５０６和－ ０．５５２）。由此提示Ⅳ型高脂血症患者的 Ｈ Ｄ Ｌ成熟过程可能受阻。
The relative percentages of serum H D L subgroups in 24 normal subjects and 24 patients with type IV hyperlipidemia were analyzed by two-dimensional electrophoresis and Western blotting. The results showed that the contents of pre-β1 H D L and H D L3a in serum were significantly increased in patients with type Ⅳ hyperlipidemia (P <0.001 and <0.05, respectively), while those in mature H D L2b levels were significantly reduced (P <0.001). Serum T G concentrations were positively correlated with pre-β1-H D L and H D L3a levels (correlation coefficients 0.582 and 0.692, respectively), but negatively correlated with H D L2b and H D L2a levels (correlation coefficient Respectively - 0.506 and - 0.552). This suggests that patients with type IV hyperlipidemia H D L mature process may be blocked.