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目的观察不同剂量丹参注射液对小鼠鼠尾表皮颗粒层形成及Th1/Th2细胞因子的影响,探讨丹参治疗银屑病的可能机制。方法用小鼠鼠尾鳞片表皮实验模型,不同组别的小鼠以丹参注射液或甲氨喋呤腹腔注射,观察其对鼠尾表皮颗粒层细胞生成的影响;ELISA法测定各组小鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)和4(IL-4)及干扰素-γ(IFN-γ)的水平。结果丹参治疗组可显著促进小鼠鼠尾表皮颗粒层细胞的形成,显著降低小鼠血清TNF-α,IL-2和IFN-γ的水平(P<0.01),其中高剂量组与MTX组差异无统计学意义(P>0.05);丹参治疗组可显著升高小鼠血清IL-4的水平,与MTX组的差异有统计学意义(P<0.01)。结论丹参可能通过调节Thl/Th2细胞因子动态平衡促进小鼠鼠尾表皮颗粒层细胞形成而发挥治疗银屑病的作用。
Objective To observe the effects of different doses of Salvia miltiorrhiza injection on the formation of granular layer and Th1 / Th2 cytokines in mouse tail epidermis, and to explore the possible mechanism of Salvia miltiorrhiza in the treatment of psoriasis. Methods Mouse mouse tail scale epidermis experimental model, different groups of mice to Salvia injection or methotrexate intraperitoneal injection to observe the rat tail epidermis granulosa cells; ELISA assay of each group of mice serum Tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2) and 4 (IL-4) and interferon-γ (IFN- γ) levels. Results Salvia miltiorrhiza treatment group could significantly promote the formation of granular layer cells in mouse tail epidermis and significantly reduce the levels of serum TNF-α, IL-2 and IFN-γ in mice (P <0.01) There was no statistical significance (P> 0.05). Salvia miltiorrhiza treatment group could significantly increase the level of serum IL-4 in mice, with statistical significance (P <0.01). Conclusion Salvia miltiorrhiza may play a role in the treatment of psoriasis by regulating the dynamic balance of Th1 / Th2 cytokine to promote the formation of granular layer cells in mouse tail epidermis.