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该文对OPG基因敲除小鼠进行micro-CT扫描,进行全身骨骼三维重建,并分析股骨结构。结果发现OPG基因敲除纯合子小鼠全身骨骼出现弥漫性蜂窝状低密度影。OPG基因敲除小鼠的骨密度、骨结构模型指数、骨小梁厚度、骨体积分数、骨表面积与体积比、连接密度以及皮质骨厚度等指标均明显小于同窝野生型小鼠,而两基因型小鼠在骨小梁数目、骨小梁分离度及各项异性度等方面无明显差异。以上数据结果表明,OPG基因敲除导致小鼠出现骨质疏松。应用micro-CT可以对小鼠骨进行无创微结构分析,较体视学方法具有一定的优势。
In this paper, OPG knockout mice were subjected to micro-CT scan, three-dimensional reconstruction of the whole body skeleton, and analysis of femoral structure. The results showed that the OPG knockout homozygous mice showed diffuse cellular skeleton of low density honeycomb. OPG knockout mice bone mineral density index, bone structure index, trabecular thickness, bone volume fraction, bone surface area and volume ratio, connection density and cortical bone thickness and other indicators were significantly smaller than that of wild-type littermates, and two There was no significant difference in the number of trabecular bone, trabecular separation, and the degree of heterosexuality in genotype mice. The above data results show that OPG knockout mice lead to osteoporosis. Micro-CT can be applied to noninvasive microstructural analysis of mouse bone, which has more advantages than stereological methods.