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采用薄层色谱分析法对复方烧伤油中黄连、黄芩进行鉴别。实验以小檗碱,黄连药材为对照品,在苯-醋酸乙酯-甲醇-异丙醇-浓氨(6:3:1.5:1.5:0.5)展开剂中展开,可见四个特征斑点;以黄芩甙、黄芩药材为对照品,在氯仿:甲醇(10:1)展开剂中展开,T=34℃,RH=57%,可见分离清晰的荧光斑点。表明此法分离效果好,具有定性检查的鉴别意义,为制订复方烧伤油的质量标准提供了实验方法和依据。
Thin-layer chromatographic analysis was used to identify Coptidis Rhizoma and Radix Astragali in compound burn oil. The experiment was conducted with berberine and Rhizoma Coptidis as reference substance, and developed in benzene-ethyl acetate-methanol-isopropanol-concentrated ammonia (6:3:1.5:1.5:0.5) developing solvent, and four characteristic spots were observed; Astragalus membranaceus and Astragalus membranaceus were used as controls and developed in a chloroform:methanol (10:1) developing solvent. T=34°C, RH=57%, and clear-cut fluorescent spots were observed. It shows that this method has good separation effect and has the distinguishing significance of qualitative inspection. It provides experimental methods and basis for formulating the quality standards of compound burn oil.