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Objectives: To study the incidence of toxoplasmosis in pregnant women in Crete and to test a designed protocol for handling those at risk of delivering congenitally infected infants. Study design: Pregnant women were screened serologically over a period of 5 years. Cases with suspected acute toxoplasmosis were treated, peripheral blood (PB), and amniotic fluid (AF)tested by polymerase chain reaction (PCR) and culture, and fetuses monitored by ultrasonography. The absence of congenital infection in infants was confirmed by serology and clinical evaluation. Results: Of the 5532 pregnant women followed, 70.57%remained seronegative, 29.45%were seropositive, and there was direct evidence of seroconversion in six cases. Acute toxoplasmosis was suspected in 185 cases, maternal parasitemia was detected in five cases and positive amniotic fluid in one case. Congenital infection was excluded in all infants followed, based on the absence of ultrasound findings in utero, lack of clinical symptoms at birth, negative Western blotting (WB) at birth and 3 months later, and descending serology for a year. Conclusion: Overall, 29.45%of the pregnant women followed were seropositive, 3.3%with suspected acute toxoplasmosis, and in 0.02%cases there was evidence of maternofetal transmission. The protocol tested allowed differentiation between acute andlatent toxoplasmosis, safe management of the cases at risk and assisted in avoidance of unwarranted pregnancy terminations.
Objectives: To study the incidence of tooplasmosis in pregnant women in Crete and to test a designed protocol for handling those at risk of delivering congenitally infected infants. Study design: Pregnant women were screened serologically over a period of 5 years. Cases with suspected acute toxoplasmosis were treated, peripheral blood (PB), and amniotic fluid (AF) tested by polymerase chain reaction (PCR) and culture, and fetuses monitored by ultrasonography. The absence of congenital infection in infants was confirmed by serology and clinical evaluation. Of the 5532 pregnant women followed, 70.57% remained seronegative, 29.45% were seropositive, and there was direct evidence of seroconversion in six cases. Acute toxoplasmosis was suspected in 185 cases, maternal parasitemia was detected in five cases and positive amniotic fluid in one case . Congenital infection was excluded in all infants followed, based on the absence of ultrasound findings in utero, lack of clinical sympto ms at birth, negative Western blotting (WB) at birth and 3 months later, and descending serology for a year. Conclusion: Overall, 29.45% of the pregnant women were were seropositive, 3.3% with suspected acute toxoplasmosis, and in 0.02% cases there was evidence of maternofetal transmission. The protocol tested allowed differentiation between acute andlatent toxoplasmosis, safe management of the cases at risk and assisted in avoidance of unwarranted pregnancy terminations.