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目的:卵巢激素对子宫内膜离子转运的影响及其可能机制。方法:采用短路电流(Isc)技术,体外测量动情期大鼠(正常对照组)及去卵巢大鼠(模型组)子宫内膜电流及电阻的变化。结果:模型组的基础Isc及跨膜电阻(R)分别为25.56±7.58μA/cm2和64.57±5.44?/cm2,均高于对照组(8.91±3.18μA/cm2;48.48±4.66?/cm2,P<0.05)。使用上皮Na+通道阻断剂阿米洛利对基础电流无明显影响;cAMP依赖的Cl-通道(CFTR)激动剂弗斯可林(10μmol/L)使Isc略有增加;CFTR阻断剂格列本脲使对照组的基础电流略有降低(P>0.05),而模型组则大幅度降低(P<0.05)。Ca2+激活的Cl-通道(CACC)阻断剂DIDS可使各组的Isc均降低,组内用药前后比较,P均<0.05;而Na+-K+-2Cl-共转运体的阻断剂布美他尼(100mmol/L)对二组Isc无明显影响。结论:去卵巢大鼠的子宫内膜的CFTR和CACC对阻断剂的敏感性均强于对照组,说明上皮离子的转运与卵巢分泌的雌、孕激素有关。
Objective: The effect of ovarian hormone on endometrial ion transport and its possible mechanism. Methods: The changes of endometrial electric current and resistance in estrus rats (normal control group) and ovariectomized rats (model group) were measured by using short-circuit current (Isc) technique. Results: The basal Isc and transmembrane resistance (R) of the model group were 25.56 ± 7.58μA / cm2 and 64.57 ± 5.44μm2 / cm2, respectively, which were significantly higher than those of the control group (8.91 ± 3.18μA / cm2, 48.48 ± 4.66μm2 / cm2, P <0.05). Amiloride, an epithelial Na + channel blocker, had no significant effect on basal currents; Isc increased slightly with cAMP-dependent Cl-channel (CFTR) agonist Forskolin (10 μmol / L); CFTR blockers The urea reduced the basal current of the control group slightly (P> 0.05), but decreased significantly in the model group (P <0.05). Ca2 + activated Cl-channel (CACC) blockers DIDS can reduce Isc in each group before and after treatment, P <0.05; and Na + -K + -2Cl-cotransporter blocker bumetan Nepalese (100mmol / L) had no significant effect on Isc in two groups. Conclusion: The sensitivity of CFTR and CACC to ovarian endometrium in ovariectomized rats is stronger than that in control group, indicating that the transport of epithelial ions is related to the secretion of estrogen and progesterone in ovary.