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目的 观察不同剂量氟化钠 ( Na F)对成骨细胞的影响及硫酸锌 ( Zn SO4 )的拮抗作用 ,为临床治疗提供实验依据。方法 取新生 2 4 h SD鼠头盖骨分离成骨细胞 ,在不同浓度的 Na F和 /或 Zn SO4( 10 -5mol/L)中培养。经 MTT法测定细胞增殖 ,细胞分化由碱性磷酸酶 ( AL P)和骨钙素水平测定得到。结果 Na F对成骨细胞增殖及分化呈双向调节 ,主要表现为大剂量抑制 ,小剂量促进。当 Na F浓度为10 -6mol/L及 10 -5mol/L时 ,细胞增殖及碱性磷酸酶活性 ( AL P)均增加 ( P<0 .0 5)。但当 Na F浓度增加至 10 -4 mol/L及 5× 10 -4 mol/L时 ,其对细胞增殖及 ALP活性呈现抑制作用。然而 ,不同剂量的 Na F对骨钙素均表现为促进其分泌 ( P<0 .0 5)。Zn SO4 与低浓度的 Na F( 10 -6mol/L、10 -5mol/L)有协同作用 ,而对高浓度的 Na F( 10 -4 mol/L、5× 10 -4 mol/L )拮抗 ,使 Na F对细胞增殖率及 ALP活性的抑制作用减弱 ,并降低高浓度 Na F所刺激的骨钙素分泌。结论 Na F对离体成骨细胞的增殖呈双向调节 ,而对细胞分化的影响呈多样性 ,早期表现为剂量依赖性双向调节 ,后期仅呈现单向刺激。 10 -5mol/L Zn SO4 拮抗大剂量 Na F,对低浓度 Na F无拮抗作用
Objective To observe the effect of different doses of sodium fluoride (NaF) on osteoblasts and the antagonism of ZnSO4, providing experimental evidence for clinical treatment. Methods The osteoblasts were isolated from newly born SD rats for 24 hours and cultured in different concentrations of NaF and / or Zn SO4 (10 -5 mol / L). Cell proliferation was measured by MTT assay, and cell differentiation was determined by alkaline phosphatase (ALP) and osteocalcin levels. Results Na F showed a bidirectional regulation of proliferation and differentiation of osteoblasts, which was mainly inhibited by high dose and promoted by low dose. When NaF concentration was 10 -6 mol / L and 10 -5 mol / L, cell proliferation and alkaline phosphatase activity (ALP) increased (P <0.05). However, when the concentration of NaF was increased to 10 -4 mol / L and 5 × 10 -4 mol / L, it inhibited cell proliferation and ALP activity. However, different doses of NaF on osteocalcin were shown to promote its secretion (P <0. 05). Zn SO4 had a synergistic effect with a low concentration of NaF (10 -6 mol / L, 10 -5 mol / L) and a high concentration of Na F (10 -4 mol / L, 5 × 10 -4 mol / L) , Weakened the inhibitory effect of NaF on cell proliferation rate and ALP activity, and decreased the secretion of osteocalcin stimulated by high concentration NaF. Conclusions NaF can regulate the proliferation of osteoblasts in vitro in a bidirectional manner. However, the effects of NaF on the differentiation of cells are diverse. Early manifestation is dose-dependent bidirectional regulation and only unidirectional stimulation at the later stage. 10 -5mol / L Zn SO4 antagonized large doses of Na F, low concentrations of Na F no antagonism